Block-staining tissues with potassium ferrocyanide-reduced osmium tetroxide and lead aspartate for electron microscopic radioautography

Journal Article · Tue May 01 00:00:00 EDT 1984 · J. Histochem. Cytochem.; (United States)

DOI:https://doi.org/10.1177/32.5.6201530· OSTI ID:6749795

Kopriwa, B M

In the hope of devising a method for prestaining tissues en bloc for electron microscopic radioautography, pieces of radioiodine-labeled liver were taken through various combinations of ferrocyanide-reduced osmium tetroxide, lead aspartate, and aqueous uranyl acetate at room temperature or at 60 degrees C. Following the tests, the method adopted for routine use was to block-stain tissues for 2 hr in potassium ferrocyanide-reduced osmium tetroxide at 4 degrees C followed by 1 hr in Walton's lead aspartate at room temperature. This simple method, which requires no manipulation before or after emulsion coating and development of the radioautographs, provides adequate contrast without inducing background fog or artifacts.

• Research Organization: Department of Anatomy, McGill University, Montreal, Quebec, Canada
• OSTI ID: 6749795
• Journal Information: J. Histochem. Cytochem.; (United States), Journal Name: J. Histochem. Cytochem.; (United States) Vol. 32:5; ISSN JHCYA
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACTINIDE COMPOUNDS
AUTORADIOGRAPHY
BODY
CHALCOGENIDES
COMPLEXES
DIGESTIVE SYSTEM
ELECTRON MICROSCOPY
FERROCYANIDES
GLANDS
HISTOLOGICAL TECHNIQUES
IODINE ISOTOPES
IRON COMPLEXES
ISOTOPES
LEAD COMPOUNDS
LIVER
MICROSCOPY
ORGANS
OSMIUM COMPOUNDS
OSMIUM OXIDES
OXIDES
OXYGEN COMPOUNDS
TRANSITION ELEMENT COMPLEXES
TRANSITION ELEMENT COMPOUNDS
URANIUM COMPOUNDS