The role of purine degradation in methane biosynthesis and energy production in Methanococcus vannielii. Progress report
Firstly, characterization of a purine degrading pathway in Methanococcus vannielii was determined. The pathway is similar to that described for Clostridia. The M. vannielli pathway differs in a few respects from the Clostridial pathway. The pathway of Clostridia uses tetrahydrofolic acid (THF), whereas the pathway of M. vannielii uses tetrahydromethanopterin (H{sub 4}MPt) as a cofactor in the transfer of both the formimino moiety of formiminoglycine and apparently in the cleavage of glycine by a glycin decarboxylase type mechanism that is dependent upon at least H{sub 4}MPt and either NAD{sup +} or NADP{sup +}. Secondly, the relationship of purine degradation to methanogenesis was investigated.
- Research Organization:
- Univ. of Kentucky Research Foundation, Lexington, KY (United States)
- Sponsoring Organization:
- USDOE Office of Energy Research, Washington, DC (United States)
- DOE Contract Number:
- FG05-89ER13995
- OSTI ID:
- 674902
- Report Number(s):
- DOE/ER/13995--T3; ON: DE99000708
- Country of Publication:
- United States
- Language:
- English
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