Contribution of nitropyrene to the mutagenic activity of coal fly ash

Journal Article · Sun Jan 01 04:00:00 EST 1984 · Environ. Mutagen.; (United States)

DOI:https://doi.org/10.1002/em.2860060204· OSTI ID:6748853

Harris, W R; Chess, E K; Okamoto, D; Remsen, J F; Later, D W

Stack-collected coal fly ash from western low-sulfur coal was extracted with 60:40 benzene/methanol. This extract was fractionated by preparative-scale high performance liquid chromatography (HPLC) and the mutagenic activity of 14 fractions was evaluated by microbial assay with salmonella typhimurium TA 1538. HPLC methods were also used to isolate 1-nitropyrene from the total benzene/methanol extract. The concentration of 1-nitropyrene in the ash extract was determined by quantitative HPLC analyses. Mutagenicity assays of the total extract and an authentic 1-nitropyrene standard with Salmonella strains TA 1538, TA 100, and TA 98 indicated that the 1-nitropyrene accounts for approximately 0.03-0.16% of the total mutagenic activity of the extract.

• Research Organization: Battelle Pacific Northwest Laboratories, Richland, WA
• DOE Contract Number: AC03-76SF00472
• OSTI ID: 6748853
• Journal Information: Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 6:2; ISSN ENMUD
• Country of Publication: United States
• Language: English

Related Subjects

01 COAL, LIGNITE, AND PEAT
016000 -- Coal
Lignite
& Peat-- Health & Safety
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
AEROSOL WASTES
AROMATICS
ASHES
BACTERIA
CARBONACEOUS MATERIALS
CHROMATOGRAPHY
COAL
CONDENSED AROMATICS
ENERGY SOURCES
EXTRACTION
FLY ASH
FOSSIL FUELS
FUELS
GAS CHROMATOGRAPHY
HYDROCARBONS
LIQUID COLUMN CHROMATOGRAPHY
MATERIALS
MICROORGANISMS
MUTAGEN SCREENING
NITRO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RESIDUES
SALMONELLA
SALMONELLA TYPHIMURIUM
SCREENING
SEPARATION PROCESSES
SOLVENT EXTRACTION
WASTES