Amino acid sequence of human cholinesterase. Annual report, 30 September 1984-30 September 1985

Technical Report · Tue Oct 01 00:00:00 EDT 1985

OSTI ID:6748737

Lockridge, O

The active-site serine residue is located 198 amino acids from the N-terminal. The active-site peptide was isolated from three different genetic types of human serum cholinesterase: from usual, atypical, and atypical-silent genotypes. It was found that the amino acid sequence of the active-site peptide was identical in all three genotypes. Comparison of the complete sequences of cholinesterase from human serum and acetylcholinesterase from the electric organ of Torpedo californica shows an identity of 53%. Cholinesterase is of interest to the Department of Defense because cholinesterase protects against organophosphate poisons of the type used in chemical warfare. The structural results presented here will serve as the basis for cloning the gene for cholinesterase. The potential uses of large amounts of cholinesterase would be for cleaning up spills of organophosphates and possibly for detoxifying exposed personnel.

• Research Organization: American Rice, Inc., Houston, TX
• OSTI ID: 6748737
• Report Number(s): AD-A-195778/6/XAB
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
CHEMICAL WARFARE AGENTS
DETOXIFICATION
CHOLINESTERASE
AMINO ACID SEQUENCE
BLOOD SERUM
GENETIC ENGINEERING
PROGRESS REPORT
CARBOXYLESTERASES
DOCUMENT TYPES
ENZYMES
ESTERASES
HYDROLASES
MOLECULAR STRUCTURE
WEAPONS
400201* - Chemical & Physicochemical Properties