Changes of reduced glutathion, glutathion reductase, and glutathione peroxidase after radiation in guinea pigs
In this series of experiments the protective action of reduced glutathion due to ionizing radiation has been studied. In the experimental group 18 guinea pigs were exposed to successive radiations of 150 rad 3 or 4 days apart. Total dose given amounted to 750 rad which is the LD50 for guinea pigs. Blood samples were taken 30 min after each exposure. The control series were sham radiated but otherwise treated identically. The cells of the removed blood samples were separated by centrifugation and were subjected to the reduced glutathion stability test. GSSGR, GPer, and LDH enzyme activities were also measured of which the latter served as a marked enzyme. It was found that LDH did not show any alteration after radiation. The reduced glutathion stability test showed a consistent but minor reduction (P greater than 0.05), in the experimental group. GSSGR enzyme activity on the other hand was reduced significantly (from 176.48 +/- 11.32 to 41.34 +/- 1.17 IU/ml of packed erythrocytes, P less than 0.001) in the same group. GPer activity showed a consistent but minor elevation during the early phase of the experimental group. It was later increased significantly beginning after 600 rad total radiation on the fourth session (P less than 0.050).
- Research Organization:
- Medical and Surgical Research Center, Hacettepe University Medical School, Ankara, Turkey
- OSTI ID:
- 6748518
- Journal Information:
- Biochem. Med.; (United States), Vol. 31:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GLUTATHIONE
BIOLOGICAL RADIATION EFFECTS
LACTATE DEHYDROGENASE
DOSE-RESPONSE RELATIONSHIPS
ENZYME ACTIVITY
GUINEA PIGS
OXIDOREDUCTASES
PEROXIDASES
RADIOPROTECTIVE SUBSTANCES
RADIOSENSITIVITY EFFECTS
ANIMALS
BIOLOGICAL EFFECTS
CARBOXYLIC ACIDS
DRUGS
ENZYMES
HEMIACETAL DEHYDROGENASES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDES
POLYPEPTIDES
PORPHYRINS
PROTEINS
RADIATION EFFECTS
RODENTS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals