Formation of glucuronide, sulphate and glutathione conjugates of benzo(a)pyrene metabolites in hepatocytes isolated from inbred strains of mice
Metabolism of benzo(a)pyrene (BP) was studied in mouse hepatocytes isolated from uninduced animals of C57BL/6 Jacobs (B6) and C/sub 3/Hf/HeHa (C3) inbred strains. Conjugates with sulfhate, glucuronate and glutathione were the major products of BP biotransformation in the intact cells. Their formation was measured by determining the radioactivity incorporated from (/sub 3/H)BP into the appropriate metabolite, after separation on silica gel TLC plates. The conjugates were identified by their susceptibility to the action of specific degrading enzymes, arylsulphatase, beta-glucuronidase and gamma-glutamyltransferase. Effects of inhibitors of conjugation galactosamine, diethyl maleate, salicylamide were also examined. Despite quantitative differences between B6 and C3 strains of mice in BP metabolism, the same degree of covalent binding of BP metabolites to cellular DNA, was observed. The results indicate a relatively high capacity of hepatocytes from uninduced mice for conjugation of BP metabolites.
- Research Organization:
- New York State Dept. of Health, Buffalo
- OSTI ID:
- 6748104
- Journal Information:
- Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 4:11; ISSN CRNGD
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
ANIMAL CELLS
ANIMALS
AROMATICS
BENZOPYRENE
BIOCHEMICAL REACTION KINETICS
BODY
CARBOXYLIC ACIDS
CELL CULTURES
CHROMATOGRAPHY
CONDENSED AROMATICS
DIGESTIVE SYSTEM
DNA
DRUGS
ENZYME ACTIVITY
GLANDS
GLUCURONIC ACID
GLUTATHIONE
HYDROCARBONS
HYDROXY ACIDS
INHIBITION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIVER
MAMMALS
METABOLISM
METABOLITES
MICE
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
REACTION KINETICS
RODENTS
SEPARATION PROCESSES
SULFATES
SULFUR COMPOUNDS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES