Bacterial production and growth rate estimation from ( sup 3 H)thymidine incorporation for attached and free-living bacteria in aquatic systems

Journal Article · Thu Feb 01 04:00:00 EST 1990 · Applied and Environmental Microbiology; (USA)

OSTI ID:6743602

Iriberri, J; Unanue, M; Ayo, B

Production and specific growth rates of attached and free-living bacteria were estimated in an oligotrophic marine system, La Salvaje Beach, Vizcaya, Spain, and in a freshwater system having a higher nutrient concentration, Butron River, Vizcaya, Spain. Production was calculated from (methyl-{sup 3}H)thymidine incorporation by estimating specific conversion factors (cells or micrograms of C produced per mole of thymidine incorporated) for attached and free-living bacteria, respectively, in each system. Conversion factors were not statistically different between attached and free-living bacteria: 6.812 {times} 10{sup 11} and 8.678 {times} 10{sup 11} {mu}g of C mol{sup {minus}1} for free-living and attached bacteria in the freshwater system, and 1.276 {times} 10{sup 11} and 1.354 {times} 10{sup 11} {mu}g of C mol{sup {minus}1} for free-living and attached bacteria in the marine system. Therefore, use of a unique conversion factor for the mixed bacterial population is well founded. However, conversion factors were higher in the freshwater system than in the marine system. This could be due to the different tropic conditions of the two systems. Free-living bacteria contributed the most to production in the two systems (85% in the marine system and 67% in the freshwater system) because of their greater contribution to total biomass. Specific growth rates calculated from production data and biomass data were similar for attached and free-living bacteria.

• OSTI ID: 6743602
• Journal Information: Applied and Environmental Microbiology; (USA), Journal Name: Applied and Environmental Microbiology; (USA) Vol. 56:2; ISSN 0099-2240; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

550701* -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AQUATIC ECOSYSTEMS
AZINES
BACTERIA
BIOMASS
DEVELOPING COUNTRIES
ECOLOGY
ECOSYSTEMS
ENERGY SOURCES
EUROPE
FRESH WATER
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
MICROORGANISMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PRODUCTIVITY
PYRIMIDINES
RENEWABLE ENERGY SOURCES
RIBOSIDES
SEAS
SPAIN
SURFACE WATERS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UPTAKE
WATER
WESTERN EUROPE