Studies on Scenedesmus acutus growth. II. Effect of autotrophic and mixotrophic growth on the amino acid and the carbohydrate composition of scenedesmus acutus

Journal Article · Tue Jun 01 04:00:00 EDT 1982 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260240606· OSTI ID:6742476

Shamala, T R; Drawert, F; Leupold, G

As a general rule an increase in carbohydrates occurs during the light phase of the cell cycle and that of protein during the dark phase, although variations were found in these components under autotrophic and mixotrophic growth conditions. The results are based on the quantitative determination of carbohydrates as trimethylsilyl (TMS) derivatives and amino acids as N-trifluoroacetyl-n-butyl (TAB) esters in algal cells cultured in light and dark periods by gas-liquid chromatogrpahy (GLC). Cells harvested during the dark period contained more amino acids as compared to similar cultures harvested during the light phase. In light, the production of amino acids of the aspartate family increased in cells cultivated with glucose and carbon dioxide. With glucose as solar carbon source, the carbohydrate content was higher in the dark than in the light period. Under continuous light conditions, in the presence of carbon dioxide, there was a decrease in the synthesis of amino acids, and supplementation with glucose or carbon dioxide led to a decrease in the carbohydrate content also. Gas-liquid chromatography analysis of the extract of the purified cell walls showed that they are made up of 0.076% carbohydrates and 0.28% amino acids on the dry weight (DW) basis of whole cells. The results on the metabolism of cells, under autotrophic and mixotrophic conditions are discussed in this article. (Refs. 17).

• Research Organization: Inst fuer Chemischetechnische Analyse and Chemische Lebensmitteltechnologie der Technischen Univ, Munchen, 8050 Freising-Weihenstephan, Federal Republic of Germany
• OSTI ID: 6742476
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 24:6; ISSN BIBIA
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
140504* -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ALGAE
AMINO ACIDS
CARBOHYDRATES
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLIC ACIDS
CHALCOGENIDES
CHROMATOGRAPHY
ELECTROMAGNETIC RADIATION
GLUCOSE
GROWTH
HEXOSES
MICROORGANISMS
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PLANTS
PROTEINS
RADIATIONS
SACCHARIDES
SCENEDESMUS
SEPARATION PROCESSES
UNICELLULAR ALGAE
VISIBLE RADIATION