Saccharification of complex cellulosic substrates by the cellulase system from Clostridium thermocellum

Journal Article · Sat May 01 04:00:00 EDT 1982 · Appl. Environ. Microbiol.; (United States)

OSTI ID:6742454

Johnson, E A; Sakajoh, M; Halliwell, G; Madia, A; Demain, A L

True cellulase activity has been demonstrated in cell-free preparations from the thermophilic anaerobe Clostridium thermocellum. Such activity depends upon the presence of CA/sub 2//sup +/ and a thiol-reducing agent of which dithiothreitol is the most promising. Under these conditions, native (cotton) and derived forms of cellulose (Avicel and filter paper) were all extensively solubilized at rates comparable with cellulase from Trichoderma reesei. Maximum activity of the Clostridium cellulase was displayed at 70 degrees C and at pH 5.7 and 6.1 on Avicel and carboxymethylcellulose, respectively. In the absence of substrate at temperatures up to 70 degrees C, carboxymethylcellulase was much more unstable than the Avicel-hydrolyzing activity. (Refs. 26).

• Research Organization: Fermentation Microbiology Lab, Dept of Nutrition and Food Science, Massachusetts Inst of Tech, Cambridge, MA 02139
• OSTI ID: 6742454
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 43:5; ISSN AEMID
• Country of Publication: United States
• Language: English

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ALKALINE EARTH METAL COMPOUNDS
BACTERIA
CALCIUM COMPOUNDS
CARBOHYDRATES
CATIONS
CELLULASE
CELLULOSE
CHARGED PARTICLES
CHEMICAL REACTIONS
CLOSTRIDIUM
CLOSTRIDIUM THERMOCELLUM
DECOMPOSITION
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
HYDROLYSIS
IONS
LYSIS
MICROORGANISMS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
POLYSACCHARIDES
REDUCING AGENTS
SACCHARIDES
SACCHARIFICATION
SOLVOLYSIS