Quantitation of IgE antibody specific for ragweed and grass allergens: binding of radiolabeled allergens by solid-phase bond IgE

Journal Article · Tue Aug 01 00:00:00 EDT 1978 · J. Allergy Clin. Immunol.; (United States)

DOI:https://doi.org/10.1016/0091-6749(78)90083-0· OSTI ID:6741568

Zeiss, C R; Levitz, D; Suszko, I M

IgE antibody specific for multiple allergens extracted from grass and ragweed pollens was measured by radioimmunoassay. The assay depends on the interaction between IgE antibody bound to a polystyrene solid phase, /sup 125/I-labeled grass allergens (GA), and ragweed allergens (RW). The binding of /sup 125/I RW by serum IgE antibody from 37 allergic patients ranged from 0.2 ng to 75 ng RW protein (P) bound per ml. This binding of /sup 125/I RW by patient's IgE was paralleled by their IgE binding of /sup 125/I antigen E (AgE), a purified allergen from ragweed pollen (r = 0.90, p less than 0.001). Inhibition of patient's IgE binding of /sup 125/I RW by highly purified AgE ranged from 25 to 85% indicated individual differences in patient's IgE response to inhaled ragweed pollen. The binding of /sup 125/I GA by serum IgE antibody from 7 grass-sensitive patients ranged from 0.6 ng GA P bound per ml to 15 ng. This assay should be useful in the study of IgE responses to environmental agents containing multiple allergens and has the advantage that other antibody classes cannot interfere with the interaction between IgE antibody and labeled allergens.

• Research Organization: Northwestern Univ., Chicago, IL
• OSTI ID: 6741568
• Journal Information: J. Allergy Clin. Immunol.; (United States), Journal Name: J. Allergy Clin. Immunol.; (United States) Vol. 62:2; ISSN JACIB
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
GAMETES
GERM CELLS
GRASS
IMMUNE SERUMS
ISOTOPE APPLICATIONS
ISOTOPES
ORGANIC COMPOUNDS
ORGANIC POLYMERS
PATIENTS
PETROCHEMICALS
PETROLEUM PRODUCTS
PLANTS
PLASTICS
POLLEN
POLYMERS
POLYOLEFINS
POLYSTYRENE
POLYVINYLS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
TRACER TECHNIQUES