DNA excision repair in ultraviolet-irradiated normal and malignantly transformed mouse epidermal cell clutures
Journal Article
·
· Cancer Res.; (United States)
OSTI ID:6738482
Pyrimidine dimer production and excision was studied in ultraviolet light (UV)-irradiated primary cultures of epidermal cells derived from perinatal mouse skin and in an in vitro malignantly transformed epidermal cell line. Dimer production increased linearly with UV dose level for both cell types. However, at any given UV dose level, there were 20% fewer thymine-containing dimers induced in the primary cultures compared to the transformed cell line. The reduced dimer yield in the primary cultures was attributed to the multilayer (three cell layers) of these cultures. The primary cultures were found to excise no more than 10% of the original dimers in a 24-hr period, while the malignantly transformed cells excised 34%. Nonsemiconservative DNA repair synthesis was also studied as a function of dimer yields in the first 3 hr after irradiation. When the levels of repair replication in both cell types were compared at equal yields of UV-induced dimers, the malignantly transformed cells exhibited a higher level of repair than did the primary epidermal cells. There was no difference in the kinetics of repair replication between the two cell types at a UV dose level of 10 J/sq m over the first 6 hr after irradiation.
- Research Organization:
- German Cancer Research Center, Heidelberg
- OSTI ID:
- 6738482
- Journal Information:
- Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 37:6; ISSN CNREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CELL CULTURES
DISEASES
DNA
ELECTROMAGNETIC RADIATION
EPIDERMIS
EPITHELIUM
MAMMALS
MICE
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RADIATIONS
RECOVERY
REPAIR
RODENTS
SKIN
TISSUES
TUMOR CELLS
ULTRAVIOLET RADIATION
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CELL CULTURES
DISEASES
DNA
ELECTROMAGNETIC RADIATION
EPIDERMIS
EPITHELIUM
MAMMALS
MICE
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RADIATIONS
RECOVERY
REPAIR
RODENTS
SKIN
TISSUES
TUMOR CELLS
ULTRAVIOLET RADIATION
VERTEBRATES