Studies on gallium accumulation in inflammatory lesions: I. Gallium uptake by human polymorphonuclear leukocytes. [/sup 67/Ga, rabbits]
The mechanism of ionic gallium-67 localization in inflammatory lesions was studied. Human polymorphonuclear leukocytes (PMN) had higher Ga-67 uptake than lymphocytes, whereas red blood cells had no affinity for Ga-67. Uptake by PMN showed temperature dependence, was independent of Ga-67 concentrations, and was not inhibited by metabolic inhibitors. However, its binding to PMN could be removed by trypsin but not by neuraminidase. These results are consistent with the hypothesis that the plasma membrane serves as a diffusion barrier and Ga-67 only binds to the surface of the PMN plasma membrane. When this membrane's permeability barrier was disrupted, as in heat-killed PMN, Ga-67 uptake increased markedly. Experimental abscesses were induced with E. coli or turpentine in rabbits. Twenty-four hours after i.v. injection, only 20 percent of Ga-67 in abscesses was in fractions containing intact PMN, cell debris or bacteria; the remainder was in a soluble, non-cellular fraction (2,500-g supernatant).
- Research Organization:
- Johns Hopkins Medical Insts., Baltimore
- OSTI ID:
- 6736790
- Journal Information:
- J. Nucl. Med.; (United States), Vol. 19:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CELL MEMBRANES
PERMEABILITY
ERYTHROCYTES
RADIONUCLIDE KINETICS
GALLIUM 67
BIOLOGICAL ACCUMULATION
LEUKOCYTES
LYMPHOCYTES
ABSCESSES
DIFFUSION
ESCHERICHIA COLI
RABBITS
UPTAKE
ANIMAL CELLS
ANIMALS
BACTERIA
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
GALLIUM ISOTOPES
INFLAMMATION
INTERMEDIATE MASS NUCLEI
ISOTOPES
MAMMALS
MEMBRANES
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
PATHOLOGICAL CHANGES
RADIOISOTOPES
SOMATIC CELLS
VERTEBRATES
560172* - Radiation Effects- Nuclide Kinetics & Toxicology- Animals- (-1987)