Studies on gallium accumulation in inflammatory lesions: I. Gallium uptake by human polymorphonuclear leukocytes. [/sup 67/Ga, rabbits]

Journal Article · Sun Jan 01 04:00:00 EST 1978 · J. Nucl. Med.; (United States)

OSTI ID:6736790

Tsan, M F; Chen, W Y; Scheffel, U; Wagner, Jr, H N

The mechanism of ionic gallium-67 localization in inflammatory lesions was studied. Human polymorphonuclear leukocytes (PMN) had higher Ga-67 uptake than lymphocytes, whereas red blood cells had no affinity for Ga-67. Uptake by PMN showed temperature dependence, was independent of Ga-67 concentrations, and was not inhibited by metabolic inhibitors. However, its binding to PMN could be removed by trypsin but not by neuraminidase. These results are consistent with the hypothesis that the plasma membrane serves as a diffusion barrier and Ga-67 only binds to the surface of the PMN plasma membrane. When this membrane's permeability barrier was disrupted, as in heat-killed PMN, Ga-67 uptake increased markedly. Experimental abscesses were induced with E. coli or turpentine in rabbits. Twenty-four hours after i.v. injection, only 20 percent of Ga-67 in abscesses was in fractions containing intact PMN, cell debris or bacteria; the remainder was in a soluble, non-cellular fraction (2,500-g supernatant).

• Research Organization: Johns Hopkins Medical Insts., Baltimore
• OSTI ID: 6736790
• Journal Information: J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 19:1; ISSN JNMEA
• Country of Publication: United States
• Language: English

Related Subjects

560172* -- Radiation Effects-- Nuclide Kinetics & Toxicology-- Animals-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ABSCESSES
ANIMAL CELLS
ANIMALS
BACTERIA
BETA DECAY RADIOISOTOPES
BIOLOGICAL ACCUMULATION
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CELL MEMBRANES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DIFFUSION
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ERYTHROCYTES
ESCHERICHIA COLI
GALLIUM 67
GALLIUM ISOTOPES
INFLAMMATION
INTERMEDIATE MASS NUCLEI
ISOTOPES
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MEMBRANES
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
PATHOLOGICAL CHANGES
PERMEABILITY
RABBITS
RADIOISOTOPES
RADIONUCLIDE KINETICS
SOMATIC CELLS
UPTAKE
VERTEBRATES