Subculturing of a polychlorinated biphenyl-dechlorinating anaerobic enrichment on solid media

Journal Article · Tue Dec 01 04:00:00 EST 1992 · Applied and Environmental Microbiology; (United States)

OSTI ID:6736121

May, H D; Boyle, A W; Price, II, W A; Blake, C K ^[1]

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1. Celgene Corp., Warren, NJ (United States)

An anaerobic culture capable of dechlorinating polychlorinated biphenyls was subcultured under strict anaerobic conditions on solid media containing sterilized river sediment. The dechlorination activity was transferred as a bacterial colony on a solid medium three times. After two transfers on solid medium, the culture was no longer methanogenic but still dechlorinated a mixture of tri- and tetrachlorobiphenyls. This demonstrates that anaerobic bacteria are responsible for the polychlorinated biphenyl dechlorination and can be grown without polychlorinated biphenyl on solid media.

• OSTI ID: 6736121
• Journal Information: Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 58:12; ISSN AEMIDF; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540210 -- Environment
Terrestrial-- Basic Studies-- (1990-)
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ANAEROBIC CONDITIONS
AROMATICS
BACTERIA
BIODEGRADATION
CHEMICAL REACTIONS
CHLORINATED AROMATIC HYDROCARBONS
CULTURE MEDIA
DECHLORINATION
DECOMPOSITION
DEHALOGENATION
ENRICHMENT
HALOGENATED AROMATIC HYDROCARBONS
MICROORGANISMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
POLLUTANTS
POLYCHLORINATED BIPHENYLS