The production and repair of aflatoxin B sub 1 -induced DNA damage

Conference · Tue May 01 00:00:00 EDT 1990

OSTI ID:6728027

Leadon, S A

To investigate the influence of function or activity of a DNA sequence on its repair, we have studied excision repair of aflatoxin B{sub 1} (AFB{sub 1})-induced damage in the nontranscribed, heterochromatic alpha DNA of monkey cells and in the metallothionein genes of human cells. In confluent cells, AFB{sub 1} adducts are produced in similar frequencies in alpha and in the rest of the DNA, but removal from alpha DNA is severely deficient, however, removal of AFB{sub 1} adducts from alpha DNA is enhanced by small doses of UV. The repair deficiencies are not observed in actively growing cells. We have also shown that there is preferential repair of AFB{sub 1} damage in active genes. AFB{sub 1} damage is efficiently repaired in the active human metallothionein (hMT) genes, but deficiently repaired in inactive hMT genes. 51 refs., 3 tabs.

• Research Organization: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
• Sponsoring Organization: DOE/ER; DOHHS
• DOE Contract Number: AC03-76SF00098
• OSTI ID: 6728027
• Report Number(s): LBL-29028; CONF-9005244-1; ON: DE90013810; CNN: CA40453
• Resource Relation: Conference: Mycotoxins, cancer and health conference, Baton Rouge, LA (USA), 17-18 May 1990
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
DNA ADDUCTS
EXCISION REPAIR
AFLATOXIN
BIOLOGICAL FUNCTIONS
CHROMATIN
DNA REPAIR
DNA REPLICATION
DNA SEQUENCING
FIBROSARCOMAS
GENES
MAN
METALLOTHIONEIN
MOLECULAR BIOLOGY
MONKEYS
NEOPLASMS
ADDUCTS
ANIMALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DISEASES
FUNCTIONS
MAMMALS
METALLOPROTEINS
NUCLEIC ACID REPLICATION
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RECOVERY
REPAIR
SARCOMAS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550200* - Biochemistry
550400 - Genetics