First nucleotide sequence of an organelle transfer: chloroplastic tRNA/sup Phe/

Journal Article · Tue Nov 30 23:00:00 EST 1976 · Cell; (United States)

OSTI ID:6725271

Chang, S H ^[1]; Brum, C K; Silberklang, M; RajBhandary, U L; Hecker, L I; Barnett, W E

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1. Louisiana State Univ., Baton Rouge

The primary sequence of phenylalanine tRNA (tRNA/sup phe/) from the chloroplasts of Euglena gracilis has been determined: pG-C-U-G-G-G-A-U-A-G-C-U-C-A-G-D-U-G-Gm-U(U*)-A-G-A-G-C-G-G-A-G-G-A-C-U-G-A-A-A*-A-psi-C-C-U-U-G-U-m/sup 7/G-Py*-C-A-C-C-A-G-T-psi-C-A-A-A-U-C-U-G-G-U-U-C-C-U-A-G-C-A-C-C-A. This represents the first nucleotide sequence determined for an organelle tRNA. As do all other tRNA/sup phe/s thus far sequenced, chloroplastic tRNA/sup phe/ contains 76 nucleotides. Both in the nature of its modified nucleotides and in its sequence (although the sequence of all known tRNA/sup phe/s is quite similar), chloroplastic tRNA/sup phe/ more closely resembles procaryotic tRNA/sup phe/s than it resembles those from the cytoplasm of eucaryotes. There are eight positions in the tRNA/sup phe/ molecule where nucleotides are invariant in procaryotes but differ from invariant nucleotides in eucaryotes; at five of these positions, chloroplastic tRNA/sup phe/ is similar to procaryotes. The possible evolutionary significance of this intermediate type of structure is discussed.

• OSTI ID: 6725271
• Journal Information: Cell; (United States), Journal Name: Cell; (United States) Vol. 9; ISSN CELLB
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ALGAE
AMINO ACIDS
BIOLOGY
BIOMASS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHLOROPLASTS
ENERGY SOURCES
EUGLENA
GENETICS
MICROORGANISMS
MOLECULAR BIOLOGY
MOLECULAR STRUCTURE
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHENYLALANINE
PLANTS
RENEWABLE ENERGY SOURCES
RNA
TRANSFER RNA
UNICELLULAR ALGAE