Lack of mutagenicity of methanogenic digester effluent in the Salmonella/microsome test

Journal Article · Tue May 01 04:00:00 EDT 1984 · Bull. Environ. Contam. Toxicol.; (United States)

DOI:https://doi.org/10.1007/BF01607530· OSTI ID:6724128

Wei, C; Oblinger, J L; Richter, M F

Kaplan Industries (Bartow, FL) has inititated a large-scale waste utilization program to dispose 500 tons/day of manure from a 20,000 head cattle feed lot. About 30% of the solid material in the manure is separated and recycled as an organic fertilizer. The liquid portion, with some suspended solids, is transferred to methane digesters. Methanogenic digester effluent is centrifuged to yield a 25% protein product that can be used as an animal feed supplement; the liquid effluent is used for agricultural irrigation. Studies on the use of municipal sewage sludge as fertilizer on farmlands have demonstrated that high levels of PCB's have been found in crops grown on PCB-contaminated sludge-amended soils. Since similar reaction products, or their metabolites may be produced from heavy metals, pesticide residues, antibiotics or residues from feed present in animal manure during anaerobic conversion of this biomass to methane, better assessment of the potential hazard of digester effluent is needed. The Ames Salmonella/microsome assay for mutagenicity has been widely applied in in vitro tests for potential carcinogenicity and mutagenicity of environmental chemicals or mixtures. In this study they used the three most sensitive strains of Salmonella typhimurium, i.e., TA 97, TA 98 and TA 100, to evaluate the possible presence of genotoxic compounds in the methanogenic digester effluent.

• Research Organization: Univ. of Florida, Gainesville
• OSTI ID: 6724128
• Journal Information: Bull. Environ. Contam. Toxicol.; (United States), Journal Name: Bull. Environ. Contam. Toxicol.; (United States) Vol. 32:5; ISSN BECTA
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
AGRICULTURAL WASTES
ALKANES
ANAEROBIC DIGESTION
BACTERIA
BIOCONVERSION
CELL CONSTITUENTS
DIGESTION
HYDROCARBONS
LIQUID WASTES
MANAGEMENT
MANURES
METHANE
METHANOGENIC BACTERIA
MICROORGANISMS
MICROSOMES
MUTAGEN SCREENING
ORGANIC COMPOUNDS
ORGANIC WASTES
ORGANOIDS
PROCESSING
SALMONELLA
SALMONELLA TYPHIMURIUM
SCREENING
SLUDGES
WASTE MANAGEMENT
WASTE PROCESSING
WASTES