Serologic test systems development. Progress report, October 1, 1978-September 30, 1979
Work has continued on the development and automation of enzyme immunoassays (EIA) for detecting diseases and toxic agents in food animals. Further evaluations were made of the Technicon Autoanalyzer II(AAII) for conducting totally automated EIAs. The problems investigated were machine carryover and assay variation. Modifications greatly reduced or eliminated carryover and produced acceptable levels of test variation. The EIA for swine trichinosis was significantly improved by the use of a new, partially purified antigen preparation. The result was improved detection of early seroconversions and reduced probability for false negatives and false positives. The amplified EIA was adapted as a diagnostic test for bovine brucellosis and studies were initiated for differentiating vaccinated and infected animals. Preliminary data indicate that the IgG/sub 1/ response may be diagnostic but further studies are necessary. Development of the EIA for detecting low molecular weight contaminants and residues in food products was also initiated. Compounds studied were the antibiotics chloramphenicol, tetracycline, and gentamicin; the mycotoxin, aflatoxin, and the shale oil toxin, 2-aminofluorene. Results indicate that chloramphenicol nonspecifically binds to antibody and interferes with antibody activity. Thus, the test is not yet satisfactory. Initial attempts to automate the gentamicin test were unsuccessful because of machine carryover but modifications of the AAII have produced encouraging preliminary data. Work is continuing on the development of EIAs for all of the compounds mentioned above. (ERB)
- Research Organization:
- Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
- DOE Contract Number:
- W-7405-ENG-36
- OSTI ID:
- 6721289
- Report Number(s):
- LA-8639-PR
- Country of Publication:
- United States
- Language:
- English
Similar Records
Serologic test systems development. Progress report, July 1, 1976--September 30, 1977
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AFLATOXIN
BIOASSAY
ANTIBIOTICS
BLOOD SERUM
IMMUNOLOGY
DIAGNOSTIC TECHNIQUES
AUTOMATION
DIAGNOSTIC USES
PARASITIC DISEASES
DIAGNOSIS
TOXINS
TRICHINOSIS
ANTIGENS
DOMESTIC ANIMALS
ENZYME ACTIVITY
IMMUNOGLOBULINS
ANIMALS
DISEASES
DRUGS
GLOBULINS
INFECTIOUS DISEASES
MATERIALS
ORGANIC COMPOUNDS
PROTEINS
TOXIC MATERIALS
USES
550600* - Medicine
550200 - Biochemistry
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551000 - Physiological Systems