Numerical variation of dark cells in normal and chemically induced hyperplastic epidermis with age of animal and efficiency of tumor promoter. [Mice]
The percentage of dark basal keratinocytes was quantitatively assessed in normal epidermis of Sencar mice before and after birth and in adult epidermis after topical application of several compounds of varying promoting efficiency. The percentage of dark keratinocytes reached a maximum at the 19th day of gestation (approx.40%) and fell abruptly after birth (approx.3%). Old animals exhibited a very low number of dark basal cells (0.2%). After topical application of the weak promoters resiniferotoxin, anthralin, ethylphenylpropiolate, and 12-deoxyphorbol-13-2,4,6-decatrienoate, the percentage of dark cells in young adult epidermis did not differ markedly from that in control (acetone-treated) specimens. The strong first-stage promoters 4-O-methyl-12-O-tetradecanoylphorbol-13-acetate and calcium ionophore A 23187, as well as the strong complete promoter 12-deoxyphorbol-13-deoxyphorbol-13-decanoate, induced the appearance of large numbers of dark keratinocytes, in a percentage similar to that seen after 12-O-tetra-decanoylphorbol-13-acetate application (approx.20%). The similarities between the dark keratinocytes seen after topical application of 12-O-tetradecanoylphorbol-13-acetate or other strong promoters and the dark cells observed in the fetal epidermis before the onset of the adult type of epidermal keratinization indicate that potent and/or first stage tumor promoters can be identified by their ability to induce cells resembling fetal-type dedifferentiated keratinocytes.
- Research Organization:
- Oak Ridge National Lab., TN
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 6721144
- Journal Information:
- Cancer Res.; (United States), Vol. 41
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
EPIDERMIS
ONCOGENIC TRANSFORMATIONS
AGE DEPENDENCE
TUMOR PROMOTERS
BIOLOGICAL EFFECTS
ANIMAL CELLS
BIOLOGICAL LOCALIZATION
HYDROCARBONS
KERATIN
PHORBOL ESTERS
ANIMAL TISSUES
BODY
CARCINOGENS
EPITHELIUM
ESTERS
ORGANIC COMPOUNDS
ORGANS
PROMOTERS
PROTEINS
SCLEROPROTEINS
SKIN
TISSUES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)