Cell density and non-equilibrium sorption effects on bacterial dispersal in groundwater microcosms
The relative importance of dispersion, physical straining, nonequilibrium sorption, and cell density on the dispersal of bacteria was examined in saturated, flow-dynamic sand columns. The bacterial breakthrough as a result of different size distributions of sand particles was followed by measuring the effluent concentration of (3)H-adenosine-labelled cells of a Bacillus sp. and an Enterobacter sp. strain suspended in ground-water. The breakthrough curves were compared with theoretical curves predicted from an advective-dispersive equilibrium sorption model (ADS), an ADS model with a first order sink term for irreversible cell reactions, a two-site model (equilibrium and nonequilibrium sorption sites), and a filtration model. Bacterial sand:water isotherms were linear in the experimental concentration range but had positive intercepts. The partition coefficients ranged from 15 to 0.4 for the Bacillus sp., and 120 to 0.4 for a Pseudomonas sp., and decreased with increasing particle size of the dominant fraction. In a kinetic study, the partition coefficient for the Enterobacter sp. in the smaller particle sand was 63 after one hour, but had decreased to 9 after 19 hours.
- Research Organization:
- Rice Univ., Houston, TX (United States). Dept. of Environmental Sciences and Engineering
- OSTI ID:
- 6719633
- Report Number(s):
- PB-93-141505/XAB; CNN: EPA-R-812808
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
540220* -- Environment
Terrestrial-- Chemicals Monitoring & Transport-- (1990-)
AQUATIC ECOSYSTEMS
BACILLUS
BACTERIA
BENCH-SCALE EXPERIMENTS
DATA
DENSITY
ECOSYSTEMS
ENVIRONMENTAL TRANSPORT
EQUATIONS
EQUILIBRIUM
FILTRATION
GROUND WATER
HYDROGEN COMPOUNDS
INFORMATION
ISOTHERMS
MASS TRANSFER
MICROCOSMS
MICROORGANISMS
NUMERICAL DATA
OXYGEN COMPOUNDS
PARTICLE SIZE
PARTICLES
PARTITION
PHYSICAL PROPERTIES
PREDICTION EQUATIONS
PSEUDOMONAS
RANGE
SAND
SEPARATION PROCESSES
SIZE
SOILS
SORPTION
THEORETICAL DATA
WATER