Cholesterol 26-hydroxylase activity of hamster liver mitochondria: Isotope ratio analysis using deuterated 26-hydroxycholesterol
Journal Article
·
· Journal of Lipid Research; (USA)
OSTI ID:6718875
- New York Univ. Medical Center, NY (USA)
Deuterated 26-hydroxycholesterol prepared from diosgenin by modifications of existing methods permitted the determination of mitochondrial cholesterol 26-hydroxylase using endogenous cholesterol as the substrate. Enzyme activity in a group of Syrian hamsters was found to be 10.3 +/- 3.7 pmol.min-1.mg protein-1.
- OSTI ID:
- 6718875
- Journal Information:
- Journal of Lipid Research; (USA), Vol. 31:4; ISSN 0022-2275
- Country of Publication:
- United States
- Language:
- English
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OSTI ID:6718875
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
HYDROXYLASES
ENZYME ACTIVITY
CHOLESTEROL
DEUTERIUM
HAMSTERS
ISOTOPE RATIO
LIVER
MITOCHONDRIA
TRACER TECHNIQUES
ANIMALS
BODY
CELL CONSTITUENTS
DIGESTIVE SYSTEM
ENZYMES
GLANDS
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
OXIDOREDUCTASES
RODENTS
STABLE ISOTOPES
STEROIDS
STEROLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
HYDROXYLASES
ENZYME ACTIVITY
CHOLESTEROL
DEUTERIUM
HAMSTERS
ISOTOPE RATIO
LIVER
MITOCHONDRIA
TRACER TECHNIQUES
ANIMALS
BODY
CELL CONSTITUENTS
DIGESTIVE SYSTEM
ENZYMES
GLANDS
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
OXIDOREDUCTASES
RODENTS
STABLE ISOTOPES
STEROIDS
STEROLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques