Retinoic acid stimulation of human dermal fibroblast proliferation is dependent on suboptimal extracellular Ca2+ concentration

Journal Article · Fri Jun 01 04:00:00 EDT 1990 · American Journal of Pathology; (USA)

OSTI ID:6718676

Varani, J; Shayevitz, J; Perry, D; Mitra, R S; Nickoloff, B J; Voorhees, J J ^[1]

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1. Univ. of Michigan Medical School, Ann Arbor (USA)

Human dermal fibroblasts failed to proliferate when cultured in medium containing 0.15 mmol/l (millimolar) Ca2+ (keratinocyte growth medium (KGM)) but did when the external Ca2+ concentration was raised to 1.4 mmol/l. All-trans retinoic acid (retinoic acid) stimulated proliferation in KGM but did not further stimulate growth in Ca2(+)-supplemented KGM. The ability of retinoic acid to stimulate proliferation was inhibited in KGM prepared without Ca2+ or prepared with 0.03 mmol/l Ca2+ and in KGM treated with 1 mmol/l ethylene-glycol-bis-(beta-aminoethyl ether)N,N'-tetra acetic acid. Using 45Ca2+ to measure Ca2+ influx and efflux, it was found that retinoic acid minimally increased Ca2+ uptake into fibroblasts. In contrast, retinoic acid treatment of fibroblasts that had been pre-equilibrated for 1 day with 45Ca2+ inhibited release of intracellular Ca2+ into the extracellular fluid. Retinoic acid also stimulated 35S-methionine incorporation into trichloroacetic acid-precipitable material but in contrast to its effect on proliferation, stimulation of 35S-methionine incorporation occurred in both high-Ca2+ and low-Ca2+ medium. These data indicate that retinoic acid stimulation of proliferation, but not protein synthesis, is dependent on the concentration of Ca2+ in the extracellular environment.

• OSTI ID: 6718676
• Journal Information: American Journal of Pathology; (USA), Journal Name: American Journal of Pathology; (USA) Vol. 136:6; ISSN AJPAA; ISSN 0002-9440
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CALCIUM 45
CALCIUM COMPOUNDS
CALCIUM ISOTOPES
CARBOXYLIC ACID ESTERS
CARBOXYLIC ACIDS
CELL CULTURES
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
CULTURE MEDIA
DAYS LIVING RADIOISOTOPES
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ESTERS
EVEN-ODD NUCLEI
FIBROBLASTS
INHIBITION
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KERATIN
KINETICS
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
MAN
METHIONINE
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RETINOIC ACID
SCLEROPROTEINS
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES
UPTAKE
VERTEBRATES