The amyloid precursor-like protein (APLP) gene maps to the long arm of human chromosome 19
- Harvard Medical School, Boston, MA (United States)
- Center for Medical Genetics, Nottingham (United Kingdom)
We have recently isolated a cDNA from a mouse brain library that encodes a protein whose predicted amino acid sequence is 42% identical and 64% similar to that of the amyloid [beta] protein precursor (APP; 16). This 653-amino-acid amyloid precursor-like protein (APLP) is similar to APP in overall structure as well as amino acid sequence. The amino acid homologies are particularly strong in three distinct regions of the proteins where the identities are 47, 54, and 56% (16). All three of these regions are also conserved in the Drosophila APP-like gene, APPL (11). Notably, 12 cysteine residues and a N -glyco-sylation site are conserved in the extracellular portion of APLP and APP, and a clathrin-binding domain is conserved in the cytoplasmic domain. The cytoplasmic domain is also conserved in a partial CDNA reported to encode an APP-like gene in rat testes (17), These data suggest that APLP and APP are members of a highly conserved gene family. A panel of DNAs from 31 human-rodent somatic cell lines of known karyotype was digested with EcoR1. These DNAs were then probed with the human APLP cDNA clone and the hybridization pattern was consistent with the assignment of the APLP locus to chromosome 19. 17 refs., 1 fig.
- OSTI ID:
- 6717062
- Journal Information:
- Genomics; (United States), Journal Name: Genomics; (United States) Vol. 15:1; ISSN GNMCEP; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMALS
ARTHROPODS
BODY
BRAIN
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
CHROMOSOMES
CYSTEINE
DIPTERA
DROSOPHILA
FLIES
FRUIT FLIES
GENETIC MAPPING
GONADS
HUMAN CHROMOSOME 19
HUMAN CHROMOSOMES
HYBRIDIZATION
INSECTS
INVERTEBRATES
KARYOTYPE
MALE GENITALS
MAPPING
MAPS
MOLECULAR STRUCTURE
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PRECURSOR
PROTEINS
RESIDUES
TESTES
THIOLS