Kupffer cell blockade prevents induction of portal venous tolerance in rat cardiac allograft transplantation

Journal Article · Tue May 01 04:00:00 EDT 1990 · Journal of Surgical Research; (USA)

DOI:https://doi.org/10.1016/0022-4804(90)90001-I· OSTI ID:6715276

Kamei, T; Callery, M P; Flye, M W ^[1]

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1. Washington Univ. School of Medicine, St. Louis, MO (USA)

Pretransplant portal venous (pv) administration of donor antigen induces allospecific partial tolerance. Although the involved mechanism has not been defined, antigen presentation by Kupffer cells (KC) in the liver is considered to be critical. We evaluated the effect of KC blockade on this pv tolerance induction in Buffalo (RT1b) rats receiving Lewis (RT1(1)) cardiac heterotopic allografts. Control rats received no treatment, while experimental animals received 25 X 10(6) ultraviolet B-irradiated (12,000 J/m2) donor spleen cells via either the iv (systemic intravenous) or the pv routes 7 days before transplantation. Gadolinium chloride (GdCl3), a rare earth metal known to inhibit KC phagocytosis, was given (7 mg/kg) 1 and 2 days before pv preimmunization. Cardiac graft prolongation was obtained by pv (MST = 13.3 +/- 1.9 days, n = 6, vs control = 7.3 +/- 0.5 days, n = 6; P less than 0.001) but not by iv preimmunization (7.7 +/- 0.7 days, n = 6, NS vs control). KC blockade abolished the pv tolerance, as indicated by abrogation of graft prolongation (PV + GdCl3 = 8.0 +/- 0.8 days, n = 6, NS vs control). These findings suggest that effective alloantigen uptake by KC in the liver is essential for the induction of pv tolerance in rat cardiac transplantation.

• OSTI ID: 6715276
• Journal Information: Journal of Surgical Research; (USA), Journal Name: Journal of Surgical Research; (USA) Vol. 48:5; ISSN 0022-4804; ISSN JSGRA
• Country of Publication: United States
• Language: English

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