Site-specific DNA repair at the nucleosome level in a yeast minichromosome
- Washington State Univ., Pullman (USA)
The rate of excision repair of UV-induced pyrimidine dimers (PDs) was measured at specific sites in each strand of a yeast minichromosome containing an active gene (URA3), a replication origin (ARS1), and positioned nucleosomes. All six PD sites analyzed in the transcribed URA3 strand were repaired more rapidly (greater than 5-fold on average) than any of the nine PD sites analyzed in the nontranscribed strand. Efficient repair also occurred in both strands of a disrupted TRP1 gene (ten PD sites), containing four unstable nucleosomes, and in a nucleosome gap at the 5' end of URA3 (two PD sites). Conversely, slow repair occurred in both strands immediately downstream of the URA3 gene (12 of 14 PD sites). This region contains the ARS1 consensus sequence, a nucleosome gap, and two stable nucleosomes. Thus, modulation of DNA repair occurs in a simple yeast minichromosome and correlates with gene expression, nucleosome stability, and (possibly) control of replication.
- OSTI ID:
- 6715211
- Journal Information:
- Cell; (USA), Vol. 61:4; ISSN 0092-8674
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PYRIMIDINE DIMERS
RADIOINDUCTION
SACCHAROMYCES CEREVISIAE
DNA REPAIR
CHROMATIN
DNA
GENE REGULATION
GENETIC RADIATION EFFECTS
NUCLEOSOMES
PLASMIDS
TRANSCRIPTION
ULTRAVIOLET RADIATION
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CONSTITUENTS
ELECTROMAGNETIC RADIATION
EUMYCOTA
FUNGI
GENETIC EFFECTS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLANTS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SACCHAROMYCES
YEASTS
560130* - Radiation Effects on Microorganisms