Interaction of putative estrogens and the estrogen receptor system in Leydig cells in the BALB/c mouse testis resulting in the initiation of DNA synthesis
Continuous administration of estrogens for 7-9 months, both steroidal and nonsteroidal, to male BALB/c mice, leads to the formation of testicular Leydig cell tumors. Three days following the subcutaneous implantation of a pellet of estrogen in cholesterol, there is a peak in the incorporation of /sup 3/H-thymidine into the DNA of the interstitial cells. These effects are hypothesized to be mediated by the estrogen receptor system in the Leydig cell. Common experimental techniques for the measurement of hormone binding, such as dextran coated charcoal treatment, proved to be impossible to employ in this system, therefore a procedure was developed using hydroxyapatite to obtain binding data. The cytosolic estrogen receptor was found to have a dissociation constant for estradiol-17..beta.. of 6.5 x 10/sup -8/ M, while that of the nuclear estrogen receptor was 1.25 x 10/sup -8/ M. Competition assays were utilized to determine the cytosolic estrogen receptor's affinity for nonsteroidal estrogens, steroidal estrogens, and triphenylethylene.
- Research Organization:
- Loma Linda Univ., CA (USA)
- OSTI ID:
- 6713000
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ESTROGENS
CHRONIC INTAKE
RECEPTORS
BIOCHEMICAL REACTION KINETICS
TRITIUM COMPOUNDS
TRACER TECHNIQUES
CELL PROLIFERATION
DNA REPLICATION
MALES
MICE
TESTES
THYMIDINE
TUMOR CELLS
ANIMAL CELLS
ANIMALS
AZINES
BODY
GONADS
HETEROCYCLIC COMPOUNDS
HORMONES
INTAKE
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MALE GENITALS
MAMMALS
MEMBRANE PROTEINS
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PYRIMIDINES
REACTION KINETICS
RIBOSIDES
RODENTS
STEROID HORMONES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques