Molecular cloning and sequence analysis of the procine transmissible gastroenteritis coronavirus genome

Thesis/Dissertation · Tue Jan 01 04:00:00 EST 1985

OSTI ID:6709354

Kapke, P A

Two approaches were taken to examine the genome structure of the porcine transmissible gastroenteritis coronavirus (TGEV). The first approach was designed to compare the degree of nucleotide sequence homology between the genomes of TGEV and the bovine enteric coronavirus (BCV), a member of an antigenically distinct coronavirus subgroup. For this study, the RNA genomes of TGEV and BCV were separately metabolically labeled with /sup 32/P-orthophosphate, and analyzed by two-dimensional T1 oligonucleotide fingerprinting. From an analysis of comigrating oligonucleotides in a mixing experiment, it was determined that TGEV and BCV share a sequence homology of approximately 91%. This degree of homology supports the notion that the two viruses, while antigenically dissimilar, arose from a common ancestor. It further raises the question of whether conserved sequences might be confined to restricted regions of the genome allowing identification of conserved functional domains. The second approach was designed to systematically examine the primary structure of the TGEV genome thereby allowing rigorous genetic analyses. For this approach, the 3' end of the 20 Kb TGEV genome was cloned and sequenced, and the properties of two genes were deduced and analyzed.

• Research Organization: Tennessee Univ., Knoxville (USA)
• OSTI ID: 6709354
• Country of Publication: United States
• Language: English

Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
DIGESTIVE SYSTEM DISEASES
DISEASES
DOMESTIC ANIMALS
ELECTROPHORESIS
ENTERITIS
GENES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LIGHT NUCLEI
MAMMALS
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS 31
PHOSPHORUS ISOTOPES
RNA
STABLE ISOTOPES
SWINE
TRACER TECHNIQUES
VERTEBRATES
VIRUSES