Retinol uptake and esterification in the rate sertoli cell

Thesis/Dissertation · Sat Dec 31 23:00:00 EST 1988

OSTI ID:6708337

Shingleton, J L

The mechanism by which Sertoli cells accumulate retinol from retinol-binding protein (RBP) and the cellular metabolism of the accumulated retinol were investigated here using primary cultures of Sertoli cells isolated from 20 day-old rats. Cells incubated with ({sup 3}H)retinol-RBP accumulated ({sup 3}H)retinol in a time- and temperature dependent manner. The rate of ({sup 3}H) retinol accumulation declined when cellular ({sup 3}H) retinol concentrations reached approximately 0.53 pmol of retinol per {mu}g of cellular DNA, equivalent to the cellular content of cellular retinol-binding protein (CRBP). Excess unlabeled retinol-RBP competed with ({sup 3}H) retinol-RBP for ({sup 3}H) retinol delivery to the cells but free retinol did not. Furthermore, free ({sup 3}H) retinol associated with Sertoli cells in a non-saturable manner. The transport constant for specific retinol accumulation from RBP was 1.9 {mu}M, suggesting that any change in the normal circulating retinol-RBP level would directly affect the rate of retinol accumulation. Competition studies and studies using labeled RBP, cellular energy inhibitors, and lysosomal poisons indicated that the specific retinol accumulation by Sertoli cells occurs by interaction with a cell-surface receptor that internalizes retinol without concomitant internalization of RBP. Extraction and HPLC analysis of the radioactivity associated with Sertoli cells after incubation with ({sup 3}H) retinol-RBP yielded retinol and retinyl esters.

• Research Organization: Vanderbilt Univ., Nashville, TN (USA)
• OSTI ID: 6708337
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOLOGICAL ACCUMULATION
CARBOXYLIC ACID ESTERS
CHEMICAL REACTIONS
CHROMATOGRAPHY
ESTERIFICATION
ESTERS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MEMBRANE TRANSPORT
METABOLISM
ORGANIC COMPOUNDS
RATS
RETINOIC ACID
RODENTS
SEPARATION PROCESSES
TEMPERATURE DEPENDENCE
TIME DEPENDENCE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UPTAKE
VERTEBRATES