Resistance of essential fatty acid-deficient rats to endotoxin-induced increases in vascular permeability

Journal Article · Fri Jun 01 00:00:00 EDT 1990 · Circulatory Shock; (USA)

OSTI ID:6707509

Li, E J; Cook, J A; Spicer, K M; Wise, W C; Rokach, J; Halushka, P V ^[1]

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1. Medical Univ. of South Carolina, Charleston (USA)

Resistance to endotoxin in essential fatty acid-deficient (EFAD) rats is associated with reduced synthesis of certain arachidonic acid metabolites. It was hypothesized that EFAD rats would manifest decreased vascular permeability changes during endotoxemia as a consequence of reduced arachidonic acid metabolism. To test this hypothesis, changes in hematocrit (HCT) and mesenteric localization rate of technetium-labeled human serum albumin (99mTc-HSA) and red blood cells (99mTc-RBC) were assessed in EFAD and normal rats using gamma-camera imaging. Thirty minutes after Salmonella enteritidis endotoxin, EFAD rats exhibited less hemoconcentration as determined by % HCT than normal rats. Endotoxin caused a less severe change in permeability index in the splanchnic region in EFAD rats than in normal rats (1.2 +/- 0.6 x 10(-3)min-1 vs. 4.9 +/- 1.7 x 10(-3)min-1 respectively, P less than 0.05). In contrast to 99mTc-HSA, mesenteric localization of 99mTc-RBC was not changed by endotoxin in control or EFAD rats. Supplementation with ethyl-arachidonic acid did not enhance susceptibility of EFAD rats to endotoxin-induced splanchnic permeability to 99mTc-HSA. Leukotrienes have been implicated as mediators of increased vascular permeability in endotoxin shock. Since LTC3 formation has been reported to be increased in EFA deficiency, we hypothesized that LTC3 may be less potent than LTC4. Thus the effect of LTC3 on mean arterial pressure and permeability was compared to LTC4 in normal rats. LTC3-induced increases in peak mean arterial pressure were less than LTC4 at 10 micrograms/kg (39 +/- 5 mm Hg vs. 58 +/- 4 mm Hg respectively, P less than 0.05) and at 20 micrograms/kg (56 +/- 4 mm Hg vs. 75 +/- 2 mm Hg respectively, P less than 0.05). LY171883 (30 mg/kg), an LTD4/E4 receptor antagonist, attenuated the pressor effect of LTC4, LTD4, and LTC3.

• OSTI ID: 6707509
• Journal Information: Circulatory Shock; (USA), Vol. 31:2; ISSN 0092-6213
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
ARACHIDONIC ACID
METABOLISM
CAPILLARIES
PERMEABILITY
CARBOXYLIC ACIDS
BIOSYNTHESIS
ALBUMINS
BACTERIAL DISEASES
BLOOD COUNT
BLOOD PRESSURE
DISEASE RESISTANCE
ERYTHROCYTES
GAMMA CAMERAS
IMAGE PROCESSING
INHIBITION
ISOMERIC NUCLEI
METABOLITES
RATS
SALMONELLA
TECHNETIUM 99
TOXINS
ANIMALS
ANTIGENS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD VESSELS
BODY
BODY FLUIDS
CAMERAS
CARDIOVASCULAR SYSTEM
DISEASES
HOURS LIVING RADIOISOTOPES
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MATERIALS
MICROORGANISMS
MONOCARBOXYLIC ACIDS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PROCESSING
PROTEINS
RADIOISOTOPES
RODENTS
SYNTHESIS
TECHNETIUM ISOTOPES
TOXIC MATERIALS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
550601* - Medicine- Unsealed Radionuclides in Diagnostics