An efficient method for loading /sup 111/In into liposomes using acetylacetone
The efficient loading of high levels of /sup 111/In into the inner aqueous compartment of liposomes can be achieved using a water-soluble lipophilic chelate, acetylacetone. The loading method involves the incubation of the acetylacetone-/sup 111/In complex with liposomes entrapping 1mM nitrilotriacetic acid. The loading process is carried out in isotonic saline, 10 mM Tris-HCI, pH 7.6, at room temperature. Results indicate that /sup 111/In ions are concentrated in the internal aqueous compartment of liposomes by acetylacetone, which mediates the transport of /sup 111/In ions across the outermost lipid bilayer of liposomes, permitting subsequent transfer to the encapsulated nitrilotriacetic acid. As much as 90% of the acetylacetone-chelated /sup 111/In becomes internalized in the aqueous compartment of liposomes and bound by entrapped nitrilotriacetic acid. Using liposomes made from bovine brain shingomyelin/cholesterol, our results indicate that the loaded liposomes retain the entrapped internally bound /sup 111/In even after incubation with serum at 37 degrees C for 24 hr.
- Research Organization:
- University of Washington, Seattle
- OSTI ID:
- 6705738
- Journal Information:
- J. Nucl. Med.; (United States), Vol. 23:9
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
INDIUM 111
DIAGNOSTIC USES
LIPOSOMES
LABELLING
RADIOPHARMACEUTICALS
CHEMICAL PREPARATION
KETONES
PH VALUE
RETENTION
BETA DECAY RADIOISOTOPES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
RADIOISOTOPES
SYNTHESIS
USES
550601* - Medicine- Unsealed Radionuclides in Diagnostics
400702 - Radiochemistry & Nuclear Chemistry- Properties of Radioactive Materials