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Predominance of the regulatory subunit of type II cAMP-dependent protein kinase in particulate fractions of several rabbit tissues

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6704652
;

The subcellular distribution of cAMP-dependent protein kianse (cA kinase) regulatory subunits (R) from various rabbit tissues has been studied using the photoaffinity probe, 8-azido-(/sup 32/P) cAMP and specific antibodies directed against the regulatory subunits (RI and RII) from type I and II cA kinases. In the rabbit liver cytosol, the photoaffinity probe labeled one major band with an apparent molecular mass of 48,000 (48 kDa) representing RI and a minor band with an apparent molecular mass of 54,000 (54 kDa) representing RII. In contrast, the nuclear, mitochondrial and microsomal fractions from rabbit liver showed the predominant labelling of 54 kDa band representing the RII. The 54 kDa protein from all of these fractions could be immunoprecipitated by RII-antibody. Furthermore, when the solubilized mitochondrial and microsomal fractions were electrophoresed and immunoblotted using RII-antibody followed by RI-antibody, the predominant labelling of 54 kDa band was observed. In addition, the particulate fractions of rabbit skeletal muscle, heart and spleen were also found to contain RII as the predominant form. These results indicate that RII is the major form of cA kinase regulatory subunit in particulate fractions of several rabbit tissues.

Research Organization:
Clinical Research Institute of Montreal, Quebec
OSTI ID:
6704652
Report Number(s):
CONF-8606151-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
AMP
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ENZYMES
GLANDS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
LIGHT NUCLEI
LIVER
MAMMALS
MICROSOMES
MITOCHONDRIA
MOLECULAR WEIGHT
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEIN STRUCTURE
PROTEINS
RABBITS
RADIOISOTOPES
TRACER TECHNIQUES
TRANSFERASES
VERTEBRATES