Mutation induction in vivo and in vitro in human T-lymphocytes by ionizing radiation
We are quantifying the induction of mutations in human T-lymphocytes following exposure to ionizing radiation. In vivo exposure effects were studied in blood samples from patients receiving radioimmunoglobulin therapy (RIT) with {sup 131}I labeled antiferritin antibodies.In vitro exposure effects were studied in isolated T-lymphocytes (G{sub 0} phase) exposed to gamma irradiation from a {sup 137}Cs source. Both in vivo and in vitro exposures of human T-lymphocytes to ionizing radiations cause the induction of hprt mutations and an increase in the fraction of mutations resulting from gross structural alterations. Consistent with an estimate of 89--210 kb average deletion size is the observation that several hprt deletion mutations also show loss of linked anonymous DNA probes. 12 refs., 1 fig., 1 tab.
- Research Organization:
- Vermont Univ., Burlington, VT (USA). Genetics Lab.
- Sponsoring Organization:
- DOE/ER; DOHHS
- DOE Contract Number:
- FG02-87ER60502
- OSTI ID:
- 6702353
- Report Number(s):
- CONF-900144--; ON: DE90014562
- Country of Publication:
- United States
- Language:
- English
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560120* -- Radiation Effects on Biochemicals
Cells
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560151 -- Radiation Effects on Animals-- Man
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL ISOTOPES
ANIMAL CELLS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
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BODY FLUIDS
CESIUM 137
CESIUM ISOTOPES
CLONING
CONNECTIVE TISSUE CELLS
DAMAGE
DAYS LIVING RADIOISOTOPES
DISEASES
DNA SEQUENCING
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
HEPATOMAS
IMMUNOLOGY
IMMUNOTHERAPY
IN VITRO
IN VIVO
INTERMEDIATE MASS NUCLEI
IODINE 131
IODINE ISOTOPES
IONIZING RADIATIONS
IRON COMPOUNDS
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LABELLING
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MEDICINE
MUTANTS
MUTATIONS
NEOPLASMS
NUCLEAR MEDICINE
NUCLEI
ODD-EVEN NUCLEI
PATIENTS
RADIATION EFFECTS
RADIATIONS
RADIOIMMUNOLOGY
RADIOIMMUNOTHERAPY
RADIOISOTOPES
RADIOLOGY
RADIOTHERAPY
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
THERAPY
TRANSITION ELEMENT COMPOUNDS
YEARS LIVING RADIOISOTOPES