Multiple mode of binding of phencyclidines: high affinity association between phencyclidine receptors in rat brain and a monovalent ion-sensitive polypeptide

Journal Article · Fri Jan 30 04:00:00 EST 1987 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(87)90303-2· OSTI ID:6702058

Haring, R; Kloog, Y; Harshak-Felixbrodt, N A; Sokolovsky, M

Two populations of phencyclidine (PCP) binding sites are shown to exist in the rat brain: a high-affinity monovalent ion-sensitive site (Kd of 10-14 nM for (/sup 3/H)TCP, (/sup 3/H)N-(1-(2-thienyl)cyclohexyl)piperidine), which exists in both the frontal cortex and the hippocampus, and a lower affinity site (Kd of 80-130 nM for (/sup 3/H)TCP) which is found in the hippocampus but not in the frontal cortex. The nature of the interactions between the ion-binding sites and the high affinity PCP receptors depend on both ligand structure (PCP or TCP) and the ion involved (K or Na). The high-affinity sites are associated with an Mr 90,000 polypeptide whose labeling by (/sup 3/H)azido phencyclidine is selectively inhibited by monovalent ions.

• Research Organization: Tel-Aviv Univ., Israel
• OSTI ID: 6702058
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 2; ISSN BBRCA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CENTRAL NERVOUS SYSTEM AGENTS
CEREBRUM
CHEMICAL BONDS
DRUGS
HALLUCINOGENS
HIPPOCAMPUS
IN VITRO
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
MOLECULAR WEIGHT
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
POLYPEPTIDES
PROTEINS
PSYCHOTROPIC DRUGS
RATS
RECEPTORS
RODENTS
TRITIUM COMPOUNDS
VERTEBRATES