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Effect of supplemental vitamin A on colon anastomotic healing in rats given preoperative irradiation

Journal Article · · Am. J. Surg.; (United States)
We studied the effect of dietary supplementation with vitamin A on the healing of colon anastomoses in irradiated bowel. Rats were divided into two groups. Those in the first group were fed a standard chow diet and those in the second group were fed the same diet supplemented with 150 IU vitamin A/g of chow. The rats were maintained on their respective diets throughout the experiment. After 7 days, half the rats in each group underwent abdominal irradiation (200 rads). Seven days later, all of the rats underwent distal colon division and anastomosis under pentobarbital anesthesia. All rats were killed 7 days postoperatively, the colons excised, and bursting strength and hydroxyproline determinations performed on both the anastomotic segment and a normal proximal segment of adjacent colon. There was a significant decrease in the bursting strength at the colon anastomosis (p less than 0.02) and in the collagen content (p less than 0.02) after preoperative irradiation. This effect was mitigated by dietary vitamin A supplementation.
Research Organization:
Albert Einstein College of Medicine, Bronx, NY
OSTI ID:
6700280
Journal Information:
Am. J. Surg.; (United States), Journal Name: Am. J. Surg.; (United States) Vol. 2; ISSN AJSUA
Country of Publication:
United States
Language:
English