Binding of stimulated human peripheral blood lymphocytes to sepharose-concanavalin A is not reversed by methyl-. cap alpha. -mannoside

Journal Article · Thu Jan 01 04:00:00 EST 1976 · Cell. Immunol.; (United States)

DOI:https://doi.org/10.1016/0008-8749(76)90143-X· OSTI ID:6700261

Norin, A J; Kato, K; Strauss, B S

Concanavalin A (con A) bound to Sepharose beads stimulates human peripheral blood lymphocytes and as with soluble con A, DNA synthesis can be prevented by the addition of methyl-..cap alpha..-mannoside (MAM) 1 hr after exposure to mitogen. In contrast to the response of cells stimulated with soluble con A, addition of MAM 24 hr after the start of incubation with Sepharose bound mitogen does not prevent a second round of DNA replication as determined in bromodeoxyuridine density transfer experiments indicating that MAM does not dissociate the Sepharose-con A responding cell complex. We infer that within 24 hr, lymphocytes develop associations with con A-Sepharose beads at non MAM dissociable sites.

• Research Organization: Univ. of Chicago
• OSTI ID: 6700261
• Journal Information: Cell. Immunol.; (United States), Journal Name: Cell. Immunol.; (United States) Vol. 24; ISSN CLIMB
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBON 14
CARBON ISOTOPES
CELL CULTURES
CELL DIVISION
CONNECTIVE TISSUE CELLS
DNA
DNA REPLICATION
ELECTRON MICROSCOPY
EVEN-EVEN NUCLEI
HETEROCYCLIC COMPOUNDS
INHIBITION
ISOTOPES
LABELLED COMPOUNDS
LEUKOCYTES
LIGHT NUCLEI
LYMPHOCYTES
MAMMALS
MAN
MICROSCOPY
MITOSIS
NUCLEI
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PYRIMIDINES
RADIOISOTOPES
RIBOSIDES
SOMATIC CELLS
STIMULATION
SYNTHESIS
THYMIDINE
VERTEBRATES
YEARS LIVING RADIOISOTOPES