Effect of high potassium on dopamine receptor activity in bovine retina
In the present study, the hypothesis that dopamine released by light caused a subsensitivity of the dopamine receptor was investigated. Bovine eyes were obtained from a slaughterhouse, and retinas were dissected in a dark room. Filter binding assays were developed to measure agonist and antagonist binding to the dopamine receptor using ({sup 3}H)dopamine and ({sup 3}H)SCH 23390, respectively, in a retinal membrane fraction. Adenylate cyclase activation was measured by the production of ({sup 32}P)cyclic AMP from {sup 32}ATP. In desensitization experiments, bovine retinas were incubated for fifteen minutes with 56 mM potassium, which also causes a release of dopamine in retinas were washed, and membranes were prepared. The stimulation of adenylate cyclase evoked by dopamine and radiolabeled agonist and antagonist binding were measured. In the receptor binding characterization studies, the dissociation constant and the maximum number of binding sites were obtained for ({sup 3}H)dopamine and ({sup 3}H)SCH 23390 binding.
- Research Organization:
- Michigan Univ., Ann Arbor, MI (USA)
- OSTI ID:
- 6699933
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ALKALI METALS
AMINES
ANIMALS
AROMATICS
ATP
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
BODY AREAS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CATTLE
CELL CONSTITUENTS
CELL MEMBRANES
CYCLASES
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
DOPAMINE
DRUGS
ELECTROMAGNETIC RADIATION
ELEMENTS
ENZYME ACTIVITY
ENZYMES
EYES
FACE
HEAD
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
LYASES
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
METALS
NEUROREGULATORS
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHENOLS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
POLYPHENOLS
POTASSIUM
PROTEINS
RADIATIONS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RETINA
RUMINANTS
SENSE ORGANS
SYMPATHOMIMETICS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
VISIBLE RADIATION