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Tissue culture modelling of microwave induced cataracts of the eye lens. Annual summary report, 1 September 1978-31 August 1979

Technical Report ·
OSTI ID:6699717
Intact rat lenses incubated in Medium 199 (M199) at 35.5 maintain their transparency for at least 9 days. When heated to various temperatures for 1 hour to model cataractogenesis by elevated temperatures, followed by 47 hours at 35.5 C, some lenses developed cataractous opacities, depending on the temperature of incubation. At 39 C slight globular degeneration of fiber cells appeared, localized in the equatorial region, with globules up to 20 microns in diameter as has been reported for glucose-induced cataracts. At 41 C the globular degeneration was deeper at the equatorial region, extending towards the nucleus and extended to the anterior and posterior of the lens. At 47 C and 50 C some very large globules were formed (ca 200 microns across) and the globular degeneration was more extensive. At higher temperatures (60 C) lenses did not become opaque. They had normal transparency and acuity, perhaps because they had been fixed, by a process similar to histological fixation. The morphological appearance of such lenses examined by scanning electron microscopy (SEM) was normal. D-alpha-tocopherol acetate (Vitamin E) when added to lenses before incubation at 41 C, prevented the globular degeneration observed at this temperature. These results indicate that the intact rat lens in M199 appears much more sensitive to elevation in temperature than does the lens in vivo.
Research Organization:
University of Western Ontario, London (Canada). Dept. of Biochemistry
OSTI ID:
6699717
Report Number(s):
AD-A-083571
Country of Publication:
United States
Language:
English

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Related Subjects

560400* -- Other Environmental Pollutant Effects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BODY
BODY AREAS
CATARACTS
CRYSTALLINE LENS
DISEASES
ELECTROMAGNETIC RADIATION
EYES
FACE
HEAD
MAMMALS
MATHEMATICAL MODELS
MICROWAVE RADIATION
ORGANS
RADIATIONS
RATS
RODENTS
SENSE ORGANS
SENSE ORGANS DISEASES
SENSITIVITY
TEMPERATURE EFFECTS
TISSUES
VERTEBRATES
VITAMIN E
VITAMINS