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Succinate-dependent energy generation and pyruvate dehydrogenase complex activity in isolated Ascaris suum mitochondria

Thesis/Dissertation ·
OSTI ID:6698518

Body wall muscle from the parasitic nematode, Ascaris suum, contain unique anaerobic mitochondria that preferentially utilize fumarate and branched-chain enoyl CoA's as terminal electron acceptors instead of oxygen. While electron transport in these organelles is well characterized, the role of oxygen in succinate-dependent phosphorylation is still not clearly defined. Therefore, the present study was designed to more fully characterize succinate metabolism in these organelles as well as the in vitro regulation of a key mitochondrial enzyme, the pyruvate dehydrogenase complex (PDC). In the absence of added adenine nucleotides, incubations in succinate resulted in substantial elevations in intramitochrondrial ATP levels, but ATP/ADP ratios were considerably higher in incubations with malate. The stimulation of phosphorylation in aerobic incubations with succinate was rotenone sensitive and appears to be Site I dependent. Increase substrate level phosphorylation, coupled to propionate formation, or additional sites of electron-transport associated ATP synthesis were not significant. Under aerobic conditions, {sup 14}CO{sub 2} evolution from 1,4-({sup 14}C)succinate was stimulated and NADH/NAD{sup +} ratios were elevated, but the formation of {sup 14}C propionate was unchanged.

Research Organization:
Toledo Univ., OH (USA)
OSTI ID:
6698518
Country of Publication:
United States
Language:
English

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Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADP
AEROBIC CONDITIONS
ANAEROBIC CONDITIONS
ASCARIDAE
ASCARIS
ASCHELMINTHES
ATP
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMICAL REACTIONS
COENZYMES
DICARBOXYLIC ACIDS
ELECTRON TRANSFER
ENZYME ACTIVITY
ENZYMES
HELMINTHS
ISOTOPE APPLICATIONS
KETO ACIDS
LABELLED COMPOUNDS
METABOLISM
MITOCHONDRIA
MONOCARBOXYLIC ACIDS
NAD
NADH2
NEMATODES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
OXIDOREDUCTASES
PARASITES
PHOSPHORYLATION
PROPIONIC ACID
PYRUVIC ACID
QUANTITY RATIO
SUCCINIC ACID
TRACER TECHNIQUES