Human decorin gene: Intron-exon junctions and chromosomal localization

Journal Article · Fri Jan 01 04:00:00 EST 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1023· OSTI ID:6697385

Vetter, U; Young, M F; Fisher, L W ^[1]; Vogel, W; Just, W ^[2]

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1. National Institutes of Health, Bethesda, MD (United States)
2. Universitat Ulm (Germany)

All of the protein-encoding exons and the 3[prime]flanking region of the human decorin gene have been cloned an partially sequenced. The locations of the intron-exon junctions within the coding portion of the gene were identical to those found for the homologous human gene, biglycan. The sizes of the introns in the decorin gene, however, were substantially larger than those of the same introns of the biglycan gene. Portions of introns 1, 2, and 3 as well as exon 1 were not found during our extensive screening process. The 5[prime] end of intron 2 was found to have an AG-rich region followed immediately by a CT-rich region. Furthermore, the 5[prime] end of intron 3 was very rich in thymidine, whereas the 3[prime] end of intron 7 was rich in adenosine. Several cDNA clones constructed from cultured human bone cell mRNA were found to contain a different sequence at the 5[prime] end compared to that previously published for mRNA from a human embryonic fibroblast cell line. We were also unable to find the alternate 3[prime] flanking region of the previously published cDNA sequence. We have mapped the human decorin gene by in situ methods to chromosome 12q2l.3. 30 refs., 3 figs., 1 tab.

• OSTI ID: 6697385
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 15:1; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ADENOSINE
AZINES
BIOLOGICAL VARIABILITY
CHROMOSOMES
CLONING
DNA SEQUENCING
GENES
GENETIC MAPPING
GENETIC VARIABILITY
GLYCOPROTEINS
HETEROCYCLIC COMPOUNDS
HUMAN CHROMOSOME 12
HUMAN CHROMOSOMES
JUNCTIONS
MAPPING
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
PYRIMIDINES
RIBOSIDES
SCREENING
STRUCTURAL CHEMICAL ANALYSIS
THYMIDINE