Human PCK1 encoding phosphoenolpyruvate carboxykinase ls located on chromosome 20q13. 2

Journal Article · Thu Dec 31 23:00:00 EST 1992 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1040· OSTI ID:6697264

Yu, Hua; Thun, R; Chandrasekharappa, S; Trent, J M; Zhang, Ji; Meisler, M H ^[1]

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1. Univ. of Michigan, Ann Arbor (United States)

Cytoplasmic liver phosphoenolpyruvate carboxykinase (GTP) (PEPCK) catalyzes a rate-limiting step in gluconeogenesis. Primers derived from the rat liver PEPCK sequence were used to amplify a portion of the human liver cDNA and to screen a YAC library of human genomic DNA. The sequences of human and rat PEPCK CDNA differed at 16% of the nucleotides compared (45/291). Analysis of a human/rodent hybrid mapping panel demonstrated concordant segregation of PCK1 with chromosome 20. Fluorescence in situ hybridization with YAC DNA further localized PCK1 to subband 20q13.2. 4refs., 1 fig. 1 tab.

• OSTI ID: 6697264
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 15:1; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
BIOSYNTHESIS
BODY
CARBOHYDRATES
CHROMOSOMES
DIGESTIVE SYSTEM
DNA
ENZYMES
FLUORESCENCE
GENETIC MAPPING
GLANDS
GLUCOSE
HEXOSES
HUMAN CHROMOSOMES
HYBRIDIZATION
LIVER
LUMINESCENCE
MAPPING
MONOSACCHARIDES
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PHOSPHOENOLPYRUVATE
PROTEINS
SACCHARIDES
SEGREGATION
SYNTHESIS