Direct effects of ethane dimethanesulphonate on epididymal function in adult rats. An in vitro demonstration
Technical Report
·
OSTI ID:6692892
It was recently demonstrated that the Leydig cell toxicant ethane dimethanesulphonate (EDS) produces multiple effects on the epididymis after a single in vivo exposure. To determine whether any of the perturbations were mediated by a direct action of the compound, we used a novel system for the coculture of epididymal epithelial cells and sperm from the caput epididymidis. This system maintains the morphologic integrity and cell polarity of the epididymal epithelial cells before and during coculture, and the sperm recovered after coculture have intact plasma and acrosomal membranes. In addition, several functions required for epididymal sperm maturation are expressed, including the secretion of protein by the epididymal epithelium, the association of secreted protein with the plasma membrane of cocultured sperm, and the acquisition of progressive motility by cocultured sperm. In vitro exposure of epididymal epithelial cells and sperm to EDS results in a significant decline in protein secretion by the epithelial cells during coculture, and in particular, a dose-dependent decline in a 36- to 38-kd protein (PI 4.0 to 4.5) and a 34- to 36-kd protein (PI 4.5 to 5.0). Moreover, these and other proteins are not recovered from the sperm membrane of cocultured sperm after EDS treatment. Finally, EDS results in a dose-dependent decline in the percentage of both motile and progressively motile sperm recovered after coculture compared with that of sperm from untreated cocultures.
- Research Organization:
- ManTech Environmental Technology, Inc., Research Triangle Park, NC (United States)
- OSTI ID:
- 6692892
- Report Number(s):
- PB-93-141281/XAB; CNN: EPA-68-02-4450
- Country of Publication:
- United States
- Language:
- English
Similar Records
Effect of nonylphenol on male reproduction: Analysis of rat epididymal biochemical markers and antioxidant defense enzymes
Synthesis and secretion of proteins by perifused caput epididymal tubules, and association of secreted proteins with spermatozoa
Cadmium inhibits testis and epididymal acidification in vivo
Journal Article
·
Fri Jun 01 00:00:00 EDT 2012
· Toxicology and Applied Pharmacology
·
OSTI ID:22215322
Synthesis and secretion of proteins by perifused caput epididymal tubules, and association of secreted proteins with spermatozoa
Journal Article
·
Thu Oct 31 23:00:00 EST 1985
· Biol. Reprod.; (United States)
·
OSTI ID:5731674
Cadmium inhibits testis and epididymal acidification in vivo
Conference
·
Fri Feb 28 23:00:00 EST 1986
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:5523991
Related Subjects
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKANES
ANIMAL TISSUES
ANIMALS
BODY
DATA
ELECTRON MICROSCOPY
EPITHELIUM
ETHANE
FUNCTIONS
GAMETES
GERM CELLS
HYDROCARBONS
IN VITRO
IN VIVO
INFORMATION
MAMMALS
MATURATION
MEMBRANE PROTEINS
MEMBRANES
MICROSCOPY
NUMERICAL DATA
ORGANIC COMPOUNDS
PLASMA
PROTEINS
RATS
RODENTS
SECRETION
SPERMATOZOA
STATISTICAL DATA
TISSUES
TOXICITY
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKANES
ANIMAL TISSUES
ANIMALS
BODY
DATA
ELECTRON MICROSCOPY
EPITHELIUM
ETHANE
FUNCTIONS
GAMETES
GERM CELLS
HYDROCARBONS
IN VITRO
IN VIVO
INFORMATION
MAMMALS
MATURATION
MEMBRANE PROTEINS
MEMBRANES
MICROSCOPY
NUMERICAL DATA
ORGANIC COMPOUNDS
PLASMA
PROTEINS
RATS
RODENTS
SECRETION
SPERMATOZOA
STATISTICAL DATA
TISSUES
TOXICITY
VERTEBRATES