Zinc and copper tolerance of Agrostis stolonifera L. in tissue culture
Callus tissue was induced from shoot meristematic tissue and root tips of a clone of the grass Agrostis stolonifera tolerant to both zinc and copper, and from a control clone tolerant to neither metal. Growth of the callus tissue on media containing zinc and copper showed that tolerance to both metals was maintained in tissue culture. The pattern of metal uptake in tissue culture resembled uptake by whole plants in that tolerant tissue took up more metal than nontolerant tissue. Plants regenerated from callus had the same copper and zinc tolerance as the original parental clones regardless of time of growth in tissue culture and shoot or root origin of the tissue. The results support previous evidence that metal tolerance is genetically determined and acts at the cellular level.
- Research Organization:
- Duke Univ., Durham, NC
- OSTI ID:
- 6689627
- Journal Information:
- Am. J. Bot.; (United States), Vol. 65:3
- Country of Publication:
- United States
- Language:
- English
Similar Records
Selenium accumulation and selenium-salt co-tolerance in five grass species. [Festuca arundinaceae; Agropyron deserorum; Buchloe dactyloides; Agrostis stolonifera; Cynodon dactylon]
Selenium accumulation and selenium-salt cotolerance in five grass species. [Festuca arundinaceae Schred. ; Agropyron desertorum Fischi; Buchloe dactyloides (Nutt. ) Engelm. ; Agrotis stolonifera; Cynodon dactylon (L. ) Pers. , Syn]
Related Subjects
COPPER
UPTAKE
TOLERANCE
GENETIC EFFECTS
ZINC
BIOLOGICAL EFFECTS
CLONE CELLS
GENETICS
GRASS
METALS
PLANT CELLS
TISSUE CULTURES
TISSUES
BIOLOGY
BIOMASS
BODY
CELL CULTURES
ELEMENTS
ENERGY SOURCES
PLANTS
RENEWABLE ENERGY SOURCES
TRANSITION ELEMENTS
560303* - Chemicals Metabolism & Toxicology- Plants- (-1987)