Effects of ozone and nitrogen dioxide on human lung proteinase inhibitors. Research report, May 1983-January 1987

Technical Report · Sat Aug 01 04:00:00 EDT 1987

OSTI ID:6687054

Johnson, D A

This study investigated the usefulness of the oxidized forms of alpha-1-proteinase inhibitor (PI) and bronchial leukocyte proteinase inhibitor (BLPI) as in vivo markers for ozone (O/sub 3/) and nitrogen dioxide (NO/sub 2/) exposure. It was concluded that the existing assays were not sufficiently sensitive as markers of O/sub 3/ exposure. In vitro studies on the exposure of proteinase inhibitors to O/sub 3/ showed that despite their differing amino-acid compositions, they displayed similar kinetics of deactivation. While the amino acids (tryptophan, methionine, tyrosine, and histidine) were found to be most susceptible to oxidation, the absence of these did not affect the deactivation kinetics, nor did the presence of stabilizing disulfide bridges in BLPI provide increased protection against O/sub 3/. Free-radical scavengers offered more protection against oxidants than against antioxidants. The studies on NO/sub 2/ were incomplete. However, it appears that the mechanisms of action of O/sub 3/ and NO/sub 2/ are similar.

• Research Organization: Health Effects Inst., Cambridge, MA (USA)
• OSTI ID: 6687054
• Report Number(s): PB-88-234422/XAB; HEI/RR-87/11
• Country of Publication: United States
• Language: English

Related Subjects

500200 -- Environment
Atmospheric-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AIR POLLUTION
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CHALCOGENIDES
DOCUMENT TYPES
ENVIRONMENTAL EXPOSURE
ENZYME INHIBITORS
LEUKOCYTES
LUNGS
MATERIALS
NITROGEN COMPOUNDS
NITROGEN DIOXIDE
NITROGEN OXIDES
ORGANS
OXIDES
OXYGEN COMPOUNDS
OZONE
POLLUTION
PROGRESS REPORT
RESPIRATORY SYSTEM