Transposon-induced nuclear mutations that alter chloroplast gene expression
The goal of this project is to use mutant phenotypes as a guide to nuclear genes that determine the timing and localization of chloroplast development The immediate goals are to identify nuclear mutants with defects in chloroplast gene expression from maize lines harboring active Mu transposons; characterize their phenotypes to determine the precise defect in gene expression; clone several of the most interesting mutations by exploiting the transposon tag; and use the clones to further define the roles of these genes in modulating chloroplast gene expression. Three mutants were described earlier that had global defects in chloroplast gene expression. We have found that two of these mutations are allelic. Both alleles have global defects in chloroplast translation initiation, as revealed by the failure to assemble chloroplast mRNAs into polysomes. We have isolated and characterized three new mutants from Mu lines that have novel defects in chloroplast RNA metabolism. We are now ready to begin the task of cloning several of these genes, by using the Mu transposon tag.
- Research Organization:
- Oregon Univ., Eugene, OR (United States). Inst. of Molecular Biology
- Sponsoring Organization:
- DOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG06-91ER20054
- OSTI ID:
- 6686800
- Report Number(s):
- DOE/ER/20054-1; ON: DE93010774
- Country of Publication:
- United States
- Language:
- English
Similar Records
Transposon-induced nuclear mutations that alter chloroplast gene expression. Annual report, September 1, 1991--August 31, 1992
Transposon-induced nuclear mutations that alter chloroplast gene expression. Annual report, September 1, 1992--April 15, 1993
Transposon tagging and molecular analysis of the maize regulatory locus opaque-2
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Wed Dec 30 23:00:00 EST 1992
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OSTI ID:10136698
Transposon-induced nuclear mutations that alter chloroplast gene expression. Annual report, September 1, 1992--April 15, 1993
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Thu Nov 12 23:00:00 EST 1987
· Science (Washington, D.C.); (United States)
·
OSTI ID:7242431
Related Subjects
09 BIOMASS FUELS
090800 -- Biomass Fuels-- Production-- (1990-)
14 SOLAR ENERGY
140505* -- Solar Energy Conversion-- Photochemical
Photobiological
& Thermochemical Conversion-- (1980-)
550200 -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
CARBON-CARBON LYASES
CARBOXY-LYASES
CELL CONSTITUENTS
CEREALS
CHLOROPLASTS
DOCUMENT TYPES
ENZYMES
ESTERASES
GENE MUTATIONS
GENE REGULATION
GRAMINEAE
HYDROLASES
LILIOPSIDA
LYASES
MAGNOLIOPHYTA
MAIZE
MEMBRANE PROTEINS
MOLECULAR BIOLOGY
MUTATIONS
NUCLEASES
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PLANTS
PROGRESS REPORT
PROTEINS
RIBOSOMES
RIBULOSE DIPHOSPHATE CARBOXYLASE
RNA PROCESSING
THYLAKOID MEMBRANE PROTEINS
TRANSPOSONS
090800 -- Biomass Fuels-- Production-- (1990-)
14 SOLAR ENERGY
140505* -- Solar Energy Conversion-- Photochemical
Photobiological
& Thermochemical Conversion-- (1980-)
550200 -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
CARBON-CARBON LYASES
CARBOXY-LYASES
CELL CONSTITUENTS
CEREALS
CHLOROPLASTS
DOCUMENT TYPES
ENZYMES
ESTERASES
GENE MUTATIONS
GENE REGULATION
GRAMINEAE
HYDROLASES
LILIOPSIDA
LYASES
MAGNOLIOPHYTA
MAIZE
MEMBRANE PROTEINS
MOLECULAR BIOLOGY
MUTATIONS
NUCLEASES
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PLANTS
PROGRESS REPORT
PROTEINS
RIBOSOMES
RIBULOSE DIPHOSPHATE CARBOXYLASE
RNA PROCESSING
THYLAKOID MEMBRANE PROTEINS
TRANSPOSONS