Genetic approach to microbial coal desulfurization
Technical Report
·
OSTI ID:6685970
- Southern Illinois Univ., Carbondale, IL (USA). Dept. of Microbiology
Naturally occurring sulfur bacteria such as Thiobacillus and Sulfolobus can remove inorganic sulfur from coal. We hope to contribute to the development of genetically engineered bacteria which can remove the organic sulfur effectively. A process which used an appropriate mixture of bacteria to remove both types of sulfur from coal should be inexpensive and produce harmless waste products. We are cloning genes from an Escherichia coli mutant with the ability to oxidize thiophene derivatives in order to enhance this ability. We are also cloning plasmid-borne genes from wild dibenzothiophene degrading strains. By putting together the best genes from various organisms we hope to construct an efficient desulfurizing bacterium. 25 refs., 3 figs., 3 tabs.
- Research Organization:
- Southern Illinois Univ., Carbondale, IL (USA). Coal Extraction and Utilization Research Center
- Sponsoring Organization:
- DOE/FE
- DOE Contract Number:
- FC22-89PC89904
- OSTI ID:
- 6685970
- Report Number(s):
- DOE/PC/89904-T15; ON: DE90016756
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
01 COAL, LIGNITE, AND PEAT
010402* -- Coal
Lignite
& Peat-- Purification & Upgrading
550400 -- Genetics
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
CARBONACEOUS MATERIALS
CELL CONSTITUENTS
CELL CULTURES
CHEMICAL REACTIONS
CLONE CELLS
CLONING
COAL
DESULFURIZATION
DNA SEQUENCING
DOCUMENT TYPES
ENERGY SOURCES
ESCHERICHIA COLI
FOSSIL FUELS
FUELS
GENETIC ENGINEERING
HETEROCYCLIC COMPOUNDS
MATERIALS
MICROORGANISMS
MUTANTS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXIDATION
PLASMIDS
PROGRESS REPORT
STRUCTURAL CHEMICAL ANALYSIS
THIONAPHTHENES
THIOPHENE
010402* -- Coal
Lignite
& Peat-- Purification & Upgrading
550400 -- Genetics
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
CARBONACEOUS MATERIALS
CELL CONSTITUENTS
CELL CULTURES
CHEMICAL REACTIONS
CLONE CELLS
CLONING
COAL
DESULFURIZATION
DNA SEQUENCING
DOCUMENT TYPES
ENERGY SOURCES
ESCHERICHIA COLI
FOSSIL FUELS
FUELS
GENETIC ENGINEERING
HETEROCYCLIC COMPOUNDS
MATERIALS
MICROORGANISMS
MUTANTS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXIDATION
PLASMIDS
PROGRESS REPORT
STRUCTURAL CHEMICAL ANALYSIS
THIONAPHTHENES
THIOPHENE