Measurement of 7-methylguanine as an estimate of the amount of dimethylnitrosamine formed following administration of aminopyrine and nitrite to rats

Journal Article · Tue Nov 01 04:00:00 EST 1983 · Cancer Res.; (United States)

OSTI ID:6683655

Gombar, C T; Zubroff, J; Strahan, G D; Magee, P N

A dose-related increase in the excretion of 7-(methyl-/sup 14/C)methylguanine ( (/sup 14/C)m7Gua) following p.o. administration of di(methyl-/sup 14/C)methylnitrosamine to rats. Urine was collected for 24 hr after di(methyl-/sup 14/C)methylnitrosamine administration, and the purines were precipitated from an aliquot of the urine with silver nitrate. Purines were released from the precipitate with HCl, and (/sup 14/C)m7Gua was quantified by chromatography on an Aminex A-6 column. The excretion of (/sup 14/C)m7Gua increased linearly with the dose of dimethylnitrosamine. This relationship was used to estimate the amount of di(methyl-/sup 14/C)methylnitrosamine formed in the reaction of (/sup 14/C)aminopyrine with sodium nitrite in rats gavaged with these compounds. The dose of dimethylnitrosamine was also estimated from the amount of alkylation of liver DNA in the same animals. These estimates usually differed by less than a factor of 2. (/sup 14/C)aminopyrine and sodium nitrite were administered. The possibility of using this assay to obtain data on nitrosation in humans is discussed.

• OSTI ID: 6683655
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 43:11; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
560305 -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL COMPOUNDS
AMINES
ANIMALS
AROMATICS
AZAARENES
BIOASSAY
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CARBON 14 COMPOUNDS
CHROMATOGRAPHY
CLEARANCE
EXCRETION
GUANINE
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INGESTION
INTAKE
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MEASURING METHODS
METABOLISM
NITRITES
NITROGEN COMPOUNDS
NITROSAMINES
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PURINES
RATS
REACTION KINETICS
RODENTS
SEPARATION PROCESSES
SODIUM COMPOUNDS
TRACER TECHNIQUES
URINE
VERTEBRATES
WASTES