In vitro study of cellular influence on 45 Ca uptake in developing rat enamel

Journal Article · Tue Mar 01 04:00:00 EST 1977 · J. Dent. Res.; (United States)

OSTI ID:6678660

Bawden, J W; Wennberg, A

The in vitro method for culture of molar teeth from eight-day-old rats, as reported in this study, appeared to sustain reasonably normal activity in the cells of the enamel organ and pulp through culture periods of four to eight hours. Inhibition of metabolic activity in the explants by addition of 5 mM iodoacetate or 2,4-dinitrophenol to the culture medium, or by heating at 70 C for 10 minutes, did not appear to affect the intensity or pattern of 45 Ca uptake in the more advanced, rapidly mineralizing areas of the enamel. Neither did stripping of the enamel organ from the surface of the enamel have a demonstrable effect in those areas. However, metabolic inhibition with 2,4-dinitrophenol, heat killing or stripping of the enamel organ resulted in increased 45 Ca uptake in newly formed enamel adjacent to the secreting ameloblasts. It is hypothesized that calcium flux into newly formed enamel matrix is controlled, in part, by movement of the calcium, which diffuses between the ameloblasts toward the enamel surface, away from the enamel through the ameloblasts.

• Research Organization: Univ. of North Carolina, Chapel Hill
• OSTI ID: 6678660
• Journal Information: J. Dent. Res.; (United States), Journal Name: J. Dent. Res.; (United States) Vol. 56:3; ISSN JDREA
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
ANIMAL CELLS
ANIMALS
AROMATICS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CALCIUM 45
CALCIUM ISOTOPES
COATINGS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DINITROPHENOL
ENAMELS
EVEN-ODD NUCLEI
HYDROXY COMPOUNDS
IN VITRO
INHIBITION
INTERMEDIATE MASS NUCLEI
ISOTOPES
MAMMALS
METABOLISM
NITRO COMPOUNDS
NUCLEI
ORAL CAVITY
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHENOLS
RADIOISOTOPES
RADIONUCLIDE KINETICS
RATS
RODENTS
TEETH
TEMPERATURE EFFECTS
TISSUE CULTURES
UPTAKE
VERTEBRATES