Microbial metabolism of haloaromatics: isolation and properties of a chlorobenzene-degrading bacterium

Journal Article · Wed Feb 01 04:00:00 EST 1984 · Appl. Environ. Microbiol.; (United States)

OSTI ID:6678039

Reineke, W; Knackmuss, H J

A chlorobenzene-degrading bacterium was isolated by continuous enrichment from a mixture of soil and sewage samples. This organism, strain WR1306, was grown in a chemostat on a mineral medium with chlorobenzene being supplied through the vapor phase with a critical D/sub c/ value at a dilution rate of 0.55 h/sup -1/. Maximum growth rates in batch culture were accomplished at substrate concentrations of less than or equal to 0.5 mM in the culture medium. During growth on chlorobenzene, stoichiometric amounts of chloride were released. Respiration data and enzyme activities in cell extracts as well as the isolation of 3-chlorocatechol from the culture fluid are consistent with the degradation of chlorobenzene via 3-chloro-cis-1,2-dihydroxycyclohexa-3,5-diene, 3-chlorocatechol, 2-chloro-cis, cis-muconate, trans-4-carboxymethylenebut-2-en-4-olide, maleylacetate, and 3-oxoadipate. 55 references.

• Research Organization: Gesellschaft fuer Strahlen- und Umweltforschung, Munich, Federal Republic of Germany
• OSTI ID: 6678039
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 47:2; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BACTERIA
BIODEGRADATION
CHEMICAL REACTIONS
CHLORINATED AROMATIC HYDROCARBONS
DECOMPOSITION
ENZYME ACTIVITY
GROWTH
HALOGENATED AROMATIC HYDROCARBONS
METABOLISM
METABOLITES
MICROORGANISMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
RESPIRATION
SEWAGE
SOILS
WASTES