Enzymatic saccharification of pretreated rice straw and biomass production

Journal Article · Wed Oct 01 04:00:00 EDT 1986 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260281008· OSTI ID:6673815

Araujo, A; D'Souza, J

A comparative study on the saccharification of pretreated rice straw was brought about by using cellulase enzyme produced by Aspergillus terreus ATCC 52430 and its mutant strain UNGI-40. The effect of enzyme and substrate concentrations on the saccharification rate at 24 and 48 were studied. A syrup with 7% sugar concentration was obtained with a 10% substrate concentration for the mutant case, whereas a syrup with 6.8% sugar concentration was obtained with 3.5 times concentrated enzyme from the wild strain. A high saccharification value was obtained with low substrate concentration; the higher the substrate concentration used, the lower the percent saccharification. The glucose content in the hydrolysate comprised 80-82% of total reducing sugars; the remainder was cellobiose and xylose together. The hydrolysate supported the growth of yeasts Candida utilis and Saccharomyces cerevisiae ATCC 52431. A biomass with a 48% protein content was obtained. The essential amino acid composition of yeast biomass was determined.

• Research Organization: Goa College of Pharmacy, Panjim, India; Goa Univ., Bambolim, India
• OSTI ID: 6673815
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 28:10; ISSN BIBIA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
AGRICULTURAL WASTES
ALDEHYDES
AMINO ACIDS
ASPERGILLUS
CANDIDA
CARBOHYDRATES
CARBOXYLIC ACIDS
CELLULASE
CEREALS
CHEMICAL REACTIONS
COMPARATIVE EVALUATIONS
DECOMPOSITION
ENZYMATIC HYDROLYSIS
ENZYMES
FUNGI
GLUCOSE
GLYCOSYL HYDROLASES
GRASS
HEXOSES
HYDROLASES
HYDROLYSIS
LYSIS
MICROORGANISMS
MONOSACCHARIDES
MUTANTS
O-GLYCOSYL HYDROLASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC WASTES
PENTOSES
PLANTS
RICE
SACCHARIDES
SACCHARIFICATION
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SOLVOLYSIS
STRAW
SUBSTRATES
WASTES
XYLOSE
YEASTS