Dechlorination of chloroform by methanosarcina strains

Journal Article · Sun Apr 01 04:00:00 EDT 1990 · Applied and Environmental Microbiology; (USA)

OSTI ID:6669340

Mikesell, M D; Boyd, S A ^[1]

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1. Michigan State Univ., East Lansing (USA)

Dehalogenation of carbon tetrachloride, chloroform, and bromoform in pure cultures of Methanosarcina sp. strain DCM and Methanosarcina mazei S6 was demonstrated. The initial dechlorination product of chloroform was methylene chloride (dichloromethane), which accumulated transiently to about 70% of the added chloroform; trace amounts of chloromethane were also detected. The amount of chloroform dechlorinated per mole of methane produced was approximately 10 times greater than the ratio observed previously for tetrachloroethene dechlorination by these strains. The production of {sup 14}CO{sub 2} from ({sup 14}C)chloroform and the absence of {sup 14}CH{sub 4} imply that processes in addition to reductive dechlorination operate.

• OSTI ID: 6669340
• Journal Information: Applied and Environmental Microbiology; (USA), Journal Name: Applied and Environmental Microbiology; (USA) Vol. 56:4; ISSN 0099-2240; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
090900* -- Biomass Fuels-- Processing-- (1990-)
ALKANES
BACTERIA
BIODEGRADATION
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CHALCOGENIDES
CHEMICAL REACTIONS
CHLORINATED ALIPHATIC HYDROCARBONS
DECHLORINATION
DECOMPOSITION
DEHALOGENATION
HALOGENATED ALIPHATIC HYDROCARBONS
HYDROCARBONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
METABOLISM
METHANE
METHANOGENIC BACTERIA
MICROORGANISMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
TRACER TECHNIQUES